Journal ArticleDOI
Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas
Paul E. Lacy,Mery Kostianovsky +1 more
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TLDR
A simple method for the isolation of intact islets from the normal rat pancreas is described, based upon disruption of the acinar parenchyma by injecting Hanks solution into the pancreatic duct system followed by incubation of the Pancreas in collagenase.Abstract:
A simple method for the isolation of intact islets from the normal rat pancreas is described. The method is based upon disruption of the acinar parenchyma by injecting Hanks solution into the pancreatic duct system followed by incubation of the pancreas in collagenase. Islets can be separated rapidly from this mixture by sedimentation. The isolated islets release insulin in vitro and appear normal by light and electron microscopy after incubation. A centrifugation method is also described for isolation of large quantities of islets for biochemical studies.read more
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Microencapsulated islets as bioartificial endocrine pancreas
Franklin Lim,Anthony M. Sun +1 more
TL;DR: The microencapsulated islets remained morphologically and functionally intact throughout long-term culture studies lasting over 15 weeks.
Journal ArticleDOI
Free fatty acids regulate insulin secretion from pancreatic beta cells through GPR40.
Yasuaki Itoh,Yuji Kawamata,Masataka Harada,Makoto Kobayashi,Ryo Fujii,Shoji Fukusumi,Kazuhiro Ogi,Masaki Hosoya,Yasuhiro Tanaka,Hiroshi Uejima,Hideyuki Tanaka,Minoru Maruyama,Rie Satoh,Shoichi Okubo,Hideki Kizawa,Hidetoshi Komatsu,Fumika Matsumura,Yuko Noguchi,Tokuyuki Shinohara,Shuji Hinuma,Yukio Fujisawa,Masahiko Fujino +21 more
TL;DR: It is shown that a G-protein-coupled receptor, GPR40, which is abundantly expressed in the pancreas, functions as a receptor for long-chain FFAs, and that long- Chain FFAs amplify glucose-stimulated insulin secretion from pancreatic β cells by activating GPR 40.
Journal ArticleDOI
Automated Method for Isolation of Human Pancreatic Islets
TL;DR: An automated method for the isolation of human pancreatic islets meets the following requirements: minimal traumatic action on the islets, continuous digestion in which the islet that are progressively liberated can be saved from further enzymatic action, high yield and purity of the isolated islets.
Journal ArticleDOI
Chronic high-fat diet in fathers programs β-cell dysfunction in female rat offspring
TL;DR: It is shown that paternal high-fat-diet (HFD) exposure programs β-cell ‘dysfunction’ in rat F1 female offspring induces increased body weight, adiposity, impaired glucose tolerance and insulin sensitivity, and the first report in mammals of non-genetic, intergenerational transmission of metabolic sequelae of a HFD from father to offspring.
Journal ArticleDOI
Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: special reference to expression of glucose transporter isoforms
Junichi Miyazaki,Kimi Araki,Eiji Yamato,Hiroshi Ikegami,Tomoichiro Asano,Yoshikazu Shibasaki,Yoshitomo Oka,Ken Ichi Yamamura +7 more
TL;DR: The MIN6 cell line will be especially useful to analyze the molecular mechanisms by which beta cells regulate insulin secretion in response to extracellular glucose concentrations, and a possible role of GT isoforms in glucose sensing by beta cells is discussed.
References
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Journal ArticleDOI
Immunoassay of Insulin: Two Antibody System: Plasma Insulin Levels of Normal, Subdiabetic and Diabetic Rats
Carl R Morgan,Arnold Lazarow +1 more
TL;DR: A two antibody system of insulin assay for immunoassay of insulin induces the production of specific nonprecipitating antibodies, both in experimental animals and in humans.
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A modified procedure for lead staining of thin sections.
TL;DR: Among several heavy metal salts tested, the above-ment ioned " lead hydroxide" of Watson seemed to be the most effective in "s ta in ing ." Exper iments with the commercial ly available product showed that this salt would give no impregna t ion when dissolved in water, or in Institute.
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Regulation of insulin secretion studied with pieces of rabbit pancreas incubated in vitro.
TL;DR: A simple system in vitro suitable for studies on the control of insulin secretion and based on the rate of release of insulin into the incubation medium from pieces of rabbit pancreas is developed.