Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey
Melike Balk,Hans G.H.J. Heilig,Miriam H. A. van Eekert,Alfons J. M. Stams,Irene C. Rijpstra,Jaap S. Sinninghe-Damsté,Willem M. de Vos,Servé W. M. Kengen +7 more
TLDR
Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H2, and CO2 and was able to grow in an atmosphere of up to 25% of CO as sole electron donor.Abstract:
A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayas, Turkey. The cells were straight to curved rods, 0.4–0.6 μm in diameter and 3.5–10 μm in length. Spores were terminal and round. The temperature range for growth was 40–80°C, with an optimum at 70°C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H2, and CO2. Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H2 and CO2 formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA–DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.read more
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13,16-Dimethyl Octacosanedioic Acid (iso-Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3
Jaap S. Sinninghe Damsté,W. Irene C. Rijpstra,Ellen C. Hopmans,Johan W.H. Weijers,Bärbel U. Foesel,Jörg Overmann,Svetlana N. Dedysh +6 more
TL;DR: Upon both acid and base hydrolyses of total cell material, the uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic acid (iso-diabolic acid) was released in substantial amounts (22 to 43% of the total fatty acids) from all of the acidobacteria studied.
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Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure
TL;DR: It is demonstrated that the presence of membrane spanning ether-lipids and methyl branches shows a striking relationship with the growth boundaries of archaea and bacteria.
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Pathways and Bioenergetics of Anaerobic Carbon Monoxide Fermentation
TL;DR: Three main types of fermentative CO metabolism can be distinguished: hydrogenogenesis, methanogenesis, and acetogenesis, generating hydrogen, methane and acetate, respectively, with emphasis on the potential enzymatic routes and bio-energetics involved.
Journal ArticleDOI
Ether- and Ester-Bound iso-Diabolic Acid and Other Lipids in Members of Acidobacteria Subdivision 4
Jaap S. Sinninghe Damsté,W. Irene C. Rijpstra,Ellen C. Hopmans,Bärbel U. Foesel,Pia K. Wüst,Jörg Overmann,Marcus Tank,Donald A. Bryant,Peter F. Dunfield,Karen M. Houghton,Matthew B. Stott +10 more
TL;DR: Examination of lipid composition of seven phylogenetically divergent strains of subdivision 4 of the Acidobacteria, a bacterial group that is commonly encountered in soil, found the presence of ether bonds in the membrane lipids does not seem to be an adaptation to temperature, because the five mesophilic isolates contained a larger amount of ether lipids than the thermophile “Ca. Chloracidobacterium thermophilum.”
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One-carbon substrate-based biohydrogen production: Microbes, mechanism, and productivity
Simon K.-M. R. Rittmann,Hyun Sook Lee,Jae Kyu Lim,Tae Wan Kim,Jung-Hyun Lee,Sung Gyun Kang,Sung Gyun Kang +6 more
TL;DR: Recent advances in the isolation of novel phylogenetic groups utilizing formate or CO, the remarkable genetic engineering that enhances H2 productivity, and the practical implementation of H2 production from C1 substrates are focused on.
References
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Journal ArticleDOI
Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.
TL;DR: Strain TMOT was able to degrade methanol to CO2 and H2 in syntrophic culture with Methanothermobacter thermautotrophicus AH or Thermodesulfovibrio yellowstonii and is related to Thermotoga subterranea andThermotoga elfii.
Journal ArticleDOI
Two membrane-associated NiFeS-carbon monoxide dehydrogenases from the anaerobic carbon-monoxide-utilizing eubacterium Carboxydothermus hydrogenoformans.
TL;DR: Two monofunctional NiFeS carbon monoxide (CO) dehydrogenases, designated CODH I andCODH II, were purified to homogeneity from the anaerobic CO-utilizing eubacterium Carboxydothermus hydrogenoformans and it is proposed that CODh I is involved in energy generation and that C ODH II serves in anabolic functions.
Journal ArticleDOI
Taxonomic Distinction of Saccharolytic Thermophilic Anaerobes: Description of Thermoanaerobacterium xylanolyticum gen. nov., sp. nov., and Thermoanaerobacterium saccharolyticum gen. nov., sp. nov.; Reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100-69 as Thermoanaerobacter brockii comb. nov., Thermoanaerobacterium thermosulfurigenes comb. nov., and Thermoanaerobacter thermohydrosulfuricus comb. nov., Respectively; and Transfer of Clostridium thermohydrosulfuricum 39E to Thermoanaerobacter ethanolicus
TL;DR: Two thermophilic, anaerobic, xylan-degrading bacteria, strains B6A-RIT (T = type strain) and LX-11T, were isolated from Frying Pan Springs in Yellowstone National Park as discussed by the authors.
Journal ArticleDOI
Thermoanaerobacter mathranii sp. nov., an ethanol-producing, extremely thermophilic anaerobic bacterium from a hot spring in Iceland.
TL;DR: Genotypic and phenotypic differences between strain A3 and other related species indicate that strain A2 can be assigned to a new species, and the name Thermoanaerobacter mathranii is proposed.
Journal ArticleDOI
Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium.
Alexander I. Slobodkin,Tatyana P. Tourova,Boris B. Kuznetsov,Nadezhda A. Kostrikina,N. A. Chernyh,Elizaveta A. Bonch-Osmolovskaya +5 more
TL;DR: On the basis of physiological properties and phylogenetic analysis, it is proposed that strain SR4T (= DSM 12299T) should be assigned to a new species, Thermoanaerobacter siderophilus sp.