Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey
Melike Balk,Hans G.H.J. Heilig,Miriam H. A. van Eekert,Alfons J. M. Stams,Irene C. Rijpstra,Jaap S. Sinninghe-Damsté,Willem M. de Vos,Servé W. M. Kengen +7 more
TLDR
Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H2, and CO2 and was able to grow in an atmosphere of up to 25% of CO as sole electron donor.Abstract:
A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayas, Turkey. The cells were straight to curved rods, 0.4–0.6 μm in diameter and 3.5–10 μm in length. Spores were terminal and round. The temperature range for growth was 40–80°C, with an optimum at 70°C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H2, and CO2. Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H2 and CO2 formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA–DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans.read more
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13,16-Dimethyl Octacosanedioic Acid (iso-Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3
Jaap S. Sinninghe Damsté,W. Irene C. Rijpstra,Ellen C. Hopmans,Johan W.H. Weijers,Bärbel U. Foesel,Jörg Overmann,Svetlana N. Dedysh +6 more
TL;DR: Upon both acid and base hydrolyses of total cell material, the uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic acid (iso-diabolic acid) was released in substantial amounts (22 to 43% of the total fatty acids) from all of the acidobacteria studied.
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Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure
TL;DR: It is demonstrated that the presence of membrane spanning ether-lipids and methyl branches shows a striking relationship with the growth boundaries of archaea and bacteria.
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Pathways and Bioenergetics of Anaerobic Carbon Monoxide Fermentation
TL;DR: Three main types of fermentative CO metabolism can be distinguished: hydrogenogenesis, methanogenesis, and acetogenesis, generating hydrogen, methane and acetate, respectively, with emphasis on the potential enzymatic routes and bio-energetics involved.
Journal ArticleDOI
Ether- and Ester-Bound iso-Diabolic Acid and Other Lipids in Members of Acidobacteria Subdivision 4
Jaap S. Sinninghe Damsté,W. Irene C. Rijpstra,Ellen C. Hopmans,Bärbel U. Foesel,Pia K. Wüst,Jörg Overmann,Marcus Tank,Donald A. Bryant,Peter F. Dunfield,Karen M. Houghton,Matthew B. Stott +10 more
TL;DR: Examination of lipid composition of seven phylogenetically divergent strains of subdivision 4 of the Acidobacteria, a bacterial group that is commonly encountered in soil, found the presence of ether bonds in the membrane lipids does not seem to be an adaptation to temperature, because the five mesophilic isolates contained a larger amount of ether lipids than the thermophile “Ca. Chloracidobacterium thermophilum.”
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One-carbon substrate-based biohydrogen production: Microbes, mechanism, and productivity
Simon K.-M. R. Rittmann,Hyun Sook Lee,Jae Kyu Lim,Tae Wan Kim,Jung-Hyun Lee,Sung Gyun Kang,Sung Gyun Kang +6 more
TL;DR: Recent advances in the isolation of novel phylogenetic groups utilizing formate or CO, the remarkable genetic engineering that enhances H2 productivity, and the practical implementation of H2 production from C1 substrates are focused on.
References
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A new family of very long chain alpha,omega-dicarboxylic acids is a major structural fatty acyl component of the membrane lipids of Thermoanaerobacter ethanolicus 39E.
TL;DR: A careful analysis of the structures of the alpha,omega-dicarboxylic acid strongly implies that the synthetic mechanism for formation is by tail-to-tail (omega)coupling of regular iso-branched fatty acids across opposite sides of the membrane.
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Thermoanaerobacter sulfurigignens sp. nov., an anaerobic thermophilic bacterium that reduces 1 M thiosulfate to elemental sulfur and tolerates 90 mM sulfite
Yong-Jin Lee,Mona Dashti,Alexander Prange,Fred A. Rainey,Manfred Rohde,William B. Whitman,Juergen Wiegel +6 more
TL;DR: The 16S rRNA gene sequence analysis indicated that the two strains belonged to the genus Thermoanaerobacter, with similarities in the range 95.6-92.7 %, and therefore, strains JW/SL-NZ824 and JW /SL- NZ826(T) represent a novel taxon, for which the name ThermoANAerobacteria sulfurigignens sp.
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Thermoanaerobacter uzonensis sp. nov., an anaerobic thermophilic bacterium isolated from a hot spring within the Uzon Caldera, Kamchatka, Far East Russia.
Isaac D. Wagner,Weidong Zhao,Chuanlun Zhang,Christopher S. Romanek,Manfred Rohde,Juergen Wiegel +5 more
TL;DR: Several strains of heterotrophic, anaerobic thermophilic bacteria were isolated from hot springs of the Uzon Caldera, Kamchatka, Far East Russia and strain JW/IW010(T) represents a novel taxon, for which the name Thermoanaerobacter uzonensis sp.
Journal ArticleDOI
Thermoanaerobacter finnii spec. nov., a New Ethanologenic Sporogenous Bacterium
TL;DR: A strain of a strictly anaerobic, thermophilic eubacterium (AKO-1) was isolated from a consortium converting acetic acid to methane at 60 °C and revealed that the organism was closely related to the non-sporeformer Thermoanaerobacter ethanolicus, however the ability to form heat resistant spores and the lack of an inner and outer cell wall layer separated the new isolate from T. ethanolicsus.
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Oligonucleotide Probes for the Detection of Representatives of the Genus Thermoanaerobacter
I. V. Subbotina,N. A. Chernyh,T. G. Sokolova,Ilya V. Kublanov,E. A. Bonch-Osmolovskaya,Alexander V. Lebedinsky +5 more
TL;DR: Based on the analysis of 16S rRNA nucleotide sequences, oligonucleotide probes were designed for the detection of representatives of the genus Thermoanaerobacter.