Journal ArticleDOI
Isolation and characterization of tyrosinase produced by marine actinobacteria and its application in the removal of phenol from aqueous environment
Suki Roy,Ishita Das,Minki Munjal,Loganathan Karthik,Gaurav Kumar,S. Satheesh Kumar,Kokati Venkata Bhaskara Rao +6 more
TLDR
The enzyme efficiently removed the phenolic compounds from aqueous solution within few hours which indicated that tyrosinase enzyme produced by Streptomyces espinosus strain LK-4 can be potently used for the removal of phenol and phenolic compound from wastewater in industries.Abstract:
The present study was focused on screening and characterization of tyrosinase enzyme produced by marine actinobacteria and its application in phenolic compounds removal from aqueous solution. A total of 20 strains were isolated from marine sediment sample and screened for tyrosinase production by using skimmed milk agar medium. Among 20 isolates, two isolates LK-4 and LK-20 showed zone of hydrolysis and these were taken for secondary screening by using tyrosine agar medium. Based on the result of secondary screening LK-4 was selected for further analysis, such as tyrosinase assay, protein content and specific activity of the enzyme. The tyrosinase enzyme was produced in a SS medium and was partially purified by ammonium sulfate precipitation, dialysis and SDS PAGE. The isolate (LK-4) was identified as Streptomyces espinosus using 16S rRNA gene sequencing and named as “Streptomyces espinosus strain LK4 (KF806735)”. The tyrosinase enzyme was immobilized in sodium alginate which was applied to remove phenolic compounds from water. The enzyme efficiently removed the phenolic compounds from aqueous solution within few hours which indicated that tyrosinase enzyme produced by Streptomyces espinosus strain LK-4 can be potently used for the removal of phenol and phenolic compounds from wastewater in industries.read more
Citations
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Journal ArticleDOI
Emerging contaminants of high concern and their enzyme-assisted biodegradation - A review.
TL;DR: Immobilization is highlighted as a promising approach to improve enzyme catalytic performance and stabilization, as well as, to protect the three-dimensional structure of the enzyme against the undesirable consequences of harsh reaction environment.
Journal ArticleDOI
Recent developments in the use of tyrosinase and laccase in environmental applications
TL;DR: This review addressed the disparity in the molecular features and catalytic mechanism of tyrosinases relevant in environmental applications and a perspective on the future use of laccases and tyros in bioremediation was discussed.
Journal ArticleDOI
Marine Actinomycetes, New Sources of Biotechnological Products
TL;DR: The use of marine actinomycetes as a potential source of antibiotics, antitumor agents, anthelmintics, and antifungals is discussed in this paper.
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Streptomyces spp. in the biocatalysis toolbox.
TL;DR: Judging by the steady increase in a number of recent references, it is clear that biocatalysts from Streptomyces spp.
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Biocatalytic membranes for combating the challenges of membrane fouling and micropollutants in water purification: A review
TL;DR: In this article, various commonly used enzymes in Biocatalytic Membrane (BCM) were discussed for removing MEC and fouling. And the scale-up, commercial viability, challenges, and future direction for improving BCMs have been discussed and shown bright possibilities for these new generation membranes.
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