Journal ArticleDOI
Isolation of predominantly submicron-sized UHMWPE wear particles from periprosthetic tissues
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TLDR
A method of tissue digestion using sodium hydroxide was applied to the isolation and recovery of ultra-high-molecular-weight polyethylene (UHMWPE) particles from tissues around failed total hip replacements to elucidate aspects of biomaterial particle size and shape that are important to the biologic response to, and clinical outcome of, total joint replacement.Abstract:
A method of tissue digestion using sodium hydroxide was applied to the isolation and recovery of ultra-high-molecular-weight polyethylene (UHMWPE) particles from tissues around failed total hip replacements. Density gradient ultracentrifugation of the digested tissues was performed to separate the UHMWPE from cell debris and other particulates. Fourier transform infrared spectroscopy and differential scanning calorimetry (DSC) verified that the recovered particles were UHMWPE. When viewed by scaning electron microscopy, individual particles were clearly observed and were either rounded or elongated. The majority were submicron in size. The application of this method to the study or particles from periprosthetic tissues may elucidate aspects of biomaterial particles size and shape that are important to the biologic response to, and clinical outcome of, total joint replacement. © 1995 John wiley & Sons, Inc.read more
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Journal ArticleDOI
Biological reactions to wear debris in total joint replacement.
Eileen Ingham,John Fisher +1 more
TL;DR: It is concluded that the pre-clinical testing of any new materials for joint replacement must include an analysis of the wear particle characteristics and their biological reactivity in addition to the usual assessment of wear.
Journal ArticleDOI
The role of macrophages in osteolysis of total joint replacement.
Eileen Ingham,John Fisher +1 more
TL;DR: Evidence is provided that bone marrow-derived macrophages may play a dual role in osteolysis associated with total joint replacement, as the major cell in host defence responding to UHMWPE particles via the production of cytokines and secondly as precursors for the osteoclasts responsible for the ensuing bone resorption.
Journal ArticleDOI
Surface grafting of artificial joints with a biocompatible polymer for preventing periprosthetic osteolysis
Toru Moro,Yoshio Takatori,Kazuhiko Ishihara,Tomohiro Konno,Yorinobu Takigawa,Tomiharu Matsushita,Ung Li Chung,Kozo Nakamura,Hiroshi Kawaguchi +8 more
TL;DR: This approach will make a major improvement in artificial joints by preventing periprosthetic osteolysis and being biologically inert by culture systems with respect to phagocytosis and resorptive cytokine secretion by macrophages.
Journal ArticleDOI
Metal wear particle characterization from metal on metal total hip replacements: Transmission electron microscopy study of periprosthetic tissues and isolated particles
Peter F. Doorn,Pat Campbell,Jack L. Worrall,Paul D. Benya,Harry A. McKellop,Harlan C. Amstutz +5 more
TL;DR: Transmission electron microscopy was used to study metal wear particles that were either in situ in cells or had been extracted from the cells by a new technique based on enzymatic tissue digestion to explain the less intense tissue reaction around metal on metal total hip replacements.
Journal Article
Biologic effects of implant debris.
Nadim J. Hallab,Joshua J. Jacobs +1 more
TL;DR: New pathways of implant debris-induced inflammatory reactions continue to be discovered, such as the "danger signaling" inflammasome pathway, which provides new targets for pharmaceutical intervention and improved implant performance.
References
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Journal ArticleDOI
Composition and morphology of wear debris in failed uncemented total hip replacement
TL;DR: Interfacial membranes collected at revision from 11 failed uncemented Ti-alloy total hip replacements were examined and polyethylene particles were found, similar to the particles seen in the base resin used in the manufacture of the acetabular implants.
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Localised endosteal bone lysis in relation to the femoral components of cemented total hip arthroplasties
TL;DR: Four cases are described of localised endosteal bone lysis in the femur occurring in association with cemented femoral components that were not obviously 'loose' radiologically and shown at operation to be related directly to a region in which there was a local defect in the cement mantle surrounding the stem.
Journal ArticleDOI
Aggressive granulomatous lesions associated with hip arthroplasty. Immunopathological studies.
TL;DR: It is suggested that granulomatosis involves an uncoupling of the normal sequence of monocyte-macrophage-mediated clearance of foreign material and tissue debris that is normally followed by fibroblast-mediated synthesis and remodeling of the extracellular matrix.
Journal ArticleDOI
Size of metallic and polyethylene debris particles in failed cemented total hip replacements.
Joong-Myung Lee,Eduardo A. Salvati,F. Betts,Edward F. DiCarlo,Stephen B. Doty,Peter G. Bullough +5 more
TL;DR: The results suggest that factors other than the size of the metal particles, such as the constituents of the alloy, and the amount and speed of generation of debris, may be more important in the failure of hip replacements.