Journal ArticleDOI
Macrophage/particle interactions: Effect of size, composition and surface area
Arun S. Shanbhag,Joshua J. Jacobs,Jonathan Black,Jonathan Black,Jorge O. Galante,Tibor T. Glant,Tibor T. Glant +6 more
TLDR
Macrophage response to particulate debris appears to be dependent on particle size, composition, and dose as given by surface area ratio, and inhibition of macrophage DNA synthesis at higher surface area ratios suggests cell damage or death.Abstract:
Particulate wear-debris are detected in histiocytes/macrophages of granulomatous tissues adjacent to loose joint prostheses. Such cell-particle interactions have been simulated in vitro by challenging macrophages with particles dosed according to weight percent, volume percent, and number of particles. Each of these dosage methods has inherent shortcomings due to varying size and density of challenging particles of different compositions. In this study we challenged P388D1 macrophages with titania and polystyrene particles (< 2 microns), with dosage based on the ratio of the surface area of the particles to the surface area of the cells. The effect of size and composition on (1) the bone resorbing activity, (2) fibroblast proliferation, and (3) secretion of IL-1 and PGE2 was determined. Macrophage response to particulate debris appears to be dependent on particle size, composition, and dose as given by surface area ratio. P388D1 macrophages challenged with titania particles released IL-1, but did not stimulate fibroblasts. Inhibition of macrophage DNA synthesis at higher surface area ratios suggests cell damage or death. Particle-stimulated cells increased bone resorption up to 125% of controls but released only basal levels of PGE2. Macrophages stimulated by wear particles are expected to synthesize numerous factors affecting events in the bone-implant interface. Using the concept of surface area ratio allows us to study and compare such cellular responses to wear particles in a standardized manner.read more
Citations
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Journal ArticleDOI
Dissemination of wear particles to the liver, spleen, and abdominal lymph nodes of patients with hip or knee replacement.
Robert M. Urban,Joshua J. Jacobs,Michael J. Tomlinson,John Gavrilovic,Jonathan Black,Michel Peoc'h +5 more
TL;DR: Systemic distribution of metallic and polyethylene wear particles was a common finding, both in patients with a previously failed implant and in those with a primary total joint prosthesis.
Journal ArticleDOI
Biological reactions to wear debris in total joint replacement.
Eileen Ingham,John Fisher +1 more
TL;DR: It is concluded that the pre-clinical testing of any new materials for joint replacement must include an analysis of the wear particle characteristics and their biological reactivity in addition to the usual assessment of wear.
Journal ArticleDOI
The role of macrophages in osteolysis of total joint replacement.
Eileen Ingham,John Fisher +1 more
TL;DR: Evidence is provided that bone marrow-derived macrophages may play a dual role in osteolysis associated with total joint replacement, as the major cell in host defence responding to UHMWPE particles via the production of cytokines and secondly as precursors for the osteoclasts responsible for the ensuing bone resorption.
Journal ArticleDOI
Metal wear particle characterization from metal on metal total hip replacements: Transmission electron microscopy study of periprosthetic tissues and isolated particles
Peter F. Doorn,Pat Campbell,Jack L. Worrall,Paul D. Benya,Harry A. McKellop,Harlan C. Amstutz +5 more
TL;DR: Transmission electron microscopy was used to study metal wear particles that were either in situ in cells or had been extracted from the cells by a new technique based on enzymatic tissue digestion to explain the less intense tissue reaction around metal on metal total hip replacements.
Journal ArticleDOI
Macrophages, Foreign Body Giant Cells and Their Response to Implantable Biomaterials
TL;DR: Having a better understanding of the role of macrophages in the tissue healing processes, especially in events that follow biomaterial implantation, can design novel biomaterials-based tissue-engineered constructs that elicit a favorable immune response upon implantation and perform for their intended applications.
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