iTRAQ-based comparative proteomic analysis in different developmental stages of Echinococcus granulosus
TLDR
In this article, the authors used the iTRAQ (isobaric tags for relative and absolute quantification) method to select differentially expressed proteins from the three consecutive developmental stages of Echinococcus granulosus : oncosphere, adult tapeworms, and protoscolex.Abstract:
Cystic echinococcosis, caused by infection with the larval stage of the cestode Echinococcus granulosus , is a chronic zoonosis. The lifecycle of the E. granulosus parasite includes three consecutive stages that require specific gene regulation or protein expression to survive environmental shifts between definitive hosts and intermediate hosts. The aim of the present study is to screen and analyze the stage differential antigens to be considered for vaccine development against E. granulosus . By using the iTRAQ (isobaric tags for relative and absolute quantification) method, the differentially expressed proteins were selected from the three consecutive developmental stages of E. granulosus : oncosphere, adult tapeworms, and protoscolex. Through a bioinformatics analysis including Clusters of Orthologous Groups (COG), Gene Ontology (GO), and pathway metabolic annotation, we identified some proteins of interest from each stage. The results showed that a large number of differentially expressed proteins (375: oncosphere vs. adult, 346: oncosphere vs. protoscolex, and 391: adult vs. protoscolex) were identified from the three main lifecycle stages. Analysis of the differential protein pathways showed that these differential proteins are mainly enriched in metabolic pathways, Huntington’s diseases, Alzheimer’s diseases, and ribosome metabolic pathways. Interestingly, among these differential proteins, expression levels of paramyosin, HSP60, HSP70, HSP90, cathepsin L1, cathepsin D, casein kinase, and calmodulin were significantly higher in the oncosphere than in the adult or protoscolex (p < 0.05). We hope our findings will help to identify potential targets for diagnosis or for therapeutic and prophylactic intervention.read more
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TL;DR: The main achievements of this process were an update of the current nomenclature of Echinococcus spp, and an agreement on a standardized description of the surgical operations for CE according to the “Approach, cyst Opening, Resection, and Completeness” framework.
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Proteomic analysis of the Echinococcus granulosus metacestode during infection of its intermediate host
Karina Mariante Monteiro,Marcos Oliveira de Carvalho,Arnaldo Zaha,Henrique Bunselmeyer Ferreira +3 more
TL;DR: A proteomic analysis of the E. granulosus metacestode during infection of its intermediate bovine host provides valuable information on parasite survival strategies in the adverse host environment and on the molecular mechanisms underpinning CHD immunopathology.
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Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces
Veridiana Gomes Virginio,Karina Mariante Monteiro,Fernanda Drumond,Marcos Oliveira de Carvalho,Daiani Machado de Vargas,Arnaldo Zaha,Henrique Bunselmeyer Ferreira +6 more
TL;DR: This work performed an LC-MS/MS proteomic analysis of the excretory-secretory products obtained from the first 48 h of an in vitro culture of the protoscoleces, revealing a set of identified proteins not previously thought to be exposed at the host-parasite interface.