L-Rhamnose-binding lectin from eggs of the Echinometra lucunter: Amino acid sequence and molecular modeling.
Rômulo Farias Carneiro,Claudener S. Teixeira,Arthur Alves de Melo,Alexandra Sampaio de Almeida,Benildo Sousa Cavada,Oscarina Viana de Sousa,Bruno A.M. Rocha,Celso Shiniti Nagano,Alexandre Holanda Sampaio +8 more
TLDR
An L-rhamnose-binding lectin isolated from eggs of the rock boring sea urchin Echinometra lucunter was able to agglutinate Gram-positive bacterial cells, suggesting its ability to recognize pathogens.About:
This article is published in International Journal of Biological Macromolecules.The article was published on 2015-07-01 and is currently open access. It has received 15 citations till now. The article focuses on the topics: Rhamnose binding & Melibiose.read more
Citations
More filters
Journal ArticleDOI
Extensive Tandem Duplication Events Drive the Expansion of the C1q-Domain-Containing Gene Family in Bivalves.
TL;DR: The observations point out that the evolutionary process behind the development of a large arsenal of C1qDC lectin-like molecules in marine bivalves is still ongoing and likely based on an unequal crossing over.
Journal ArticleDOI
Purification, Biochemical Characterization, and Amino Acid Sequence of a Novel Type of Lectin from Aplysia dactylomela Eggs with Antibacterial/Antibiofilm Potential
Rômulo Farias Carneiro,Renato Cézar Farias Torres,Renata Pinheiro Chaves,Mayron Alves de Vasconcelos,Bruno Lopes de Sousa,André Castelo Rodrigues Goveia,Francisco Vassiliepe Sousa Arruda,Maria Nágila Carneiro Matos,Helena Matthews-Cascon,Valder N. Freire,Edson Holanda Teixeira,Celso Shiniti Nagano,Alexandre Holanda Sampaio +12 more
TL;DR: A new lectin from Aplysia dactylomela eggs (ADEL) was isolated by affinity chromatography on HCl-activated Sepharose™ media and revealed favorable binding energies for interactions between lectin and galacturonic acid, lactose, galactosamine, and Galactose.
Journal ArticleDOI
Structural analysis and anthelmintic activity of Canavalia brasiliensis lectin reveal molecular correlation between the carbohydrate recognition domain and glycans of Haemonchus contortus.
Karla L.R. Batista,Carolina R. Silva,Valdenice F. Santos,Rafael C. Silva,Renato R. Roma,Ana L.E. Santos,Raquel O. Pereira,Plínio Delatorre,Bruno A.M. Rocha,Alexandra Martins dos Santos Soares,Lívio Martins Costa-Júnior,Claudener S. Teixeira +11 more
TL;DR: These findings suggest that the inhibition of H. contortus larval development is directly related to the recognition of the core trimannoside present in the glycans of these parasites.
Journal ArticleDOI
Structural analysis, molecular docking and molecular dynamics of an edematogenic lectin from Centrolobium microchaete seeds.
Antonio Hadson Bastos Neco,Vanir Reis Pinto-Junior,David Alencar Araripe,Mayara Queiroz Santiago,Vinicius Jose Da Silva Osterne,Claudia Figueiredo Lossio,Clareane Avelino Simplicio Nobre,Messias Vital Oliveira,Mayara Torquato Lima Silva,Maria Gleiciane de Queiroz Martins,Joao Batista Cajazeiras,Gabriela Fernandes Oliveira Marques,D. R. da Costa,Kyria S. Nascimento,Ana Maria Sampaio Assreuy,Ana Maria Sampaio Assreuy,Benildo Sousa Cavada +16 more
TL;DR: The present work aimed to establish and characterize the three-dimensional structure of Centrolobium microchaete lectin by homology modeling, investigate protein-carbohydrate interactions and evaluate its inflammatory effect on mice, revealing novel interactions for mannose-specific Dalbergieae lectins.
Journal ArticleDOI
Isolation, biochemical characterization and antibiofilm effect of a lectin from the marine sponge Aplysina lactuca
Rômulo Farias Carneiro,Paulo Henrique Pinheiro de Lima,Renata Pinheiro Chaves,Rafael Pereira,Anna Luísa Pereira,Mayron Alves de Vasconcelos,Ulisses Pinheiro,Edson Holanda Teixeira,Celso Shiniti Nagano,Alexandre Holanda Sampaio +9 more
TL;DR: A new lectin was isolated from the marine sponge Aplysina lactuca by combining ammonium sulfate precipitation and affinity chromatography on guar gum matrix and it were able to significantly reduce the biomass of the bacterial biofilm tested at dose- dependent effect.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
I-TASSER: a unified platform for automated protein structure and function prediction
TL;DR: The iterative threading assembly refinement (I-TASSER) server is an integrated platform for automated protein structure and function prediction based on the sequence- to-structure-to-function paradigm.
Related Papers (5)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Purification, Biochemical Characterization, and Amino Acid Sequence of a Novel Type of Lectin from Aplysia dactylomela Eggs with Antibacterial/Antibiofilm Potential
Rômulo Farias Carneiro,Renato Cézar Farias Torres,Renata Pinheiro Chaves,Mayron Alves de Vasconcelos,Bruno Lopes de Sousa,André Castelo Rodrigues Goveia,Francisco Vassiliepe Sousa Arruda,Maria Nágila Carneiro Matos,Helena Matthews-Cascon,Valder N. Freire,Edson Holanda Teixeira,Celso Shiniti Nagano,Alexandre Holanda Sampaio +12 more