Journal ArticleDOI
Layer-by-Layer Thin Films for Co-Delivery of TGF-β siRNA and Epidermal Growth Factor to Improve Excisional Wound Healing.
Praveen Kumar Mandapalli,Suman Labala,Anup Jose,Shubhmita Bhatnagar,Renuka Janupally,Dharmarajan Sriram,Venkata Vamsi Krishna Venuganti +6 more
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TLDR
In this paper, a layer-by-layer (LbL) polyelectrolyte multilayer films containing epidermal growth factor (EGF) and TGF-β siRNA were fabricated to improve excisional wound healing and decrease scar formation.Abstract:
The major challenge with treatment of dermal wounds is accelerating healing process, while preventing the scar formation. Herein, we have fabricated layer-by-layer (LbL) polyelectrolyte multilayer films containing epidermal growth factor (EGF) and TGF-β siRNA to improve excisional wound healing and decrease scar formation. The chitosan and sodium alginate LbL thin films showed 13.0 MPa tensile strength and 2.22 N/cm2 skin adhesion strength. The LbL thin films were found to be cytocompatible, where A431 epidermal keratinocytes adhered to the film and showed 86.2 ± 0.8% cell growth compared with cells cultured in the absence of LbL thin film. In contrast, LbL thin film did not promote the Escherichia coli and Staphylococcus aureus bacterial colony formation. In a C57BL/6 mouse excisional wound model, application of LbL thin films containing TGF-β siRNA significantly (p < 0.05) reduced the TGF-β protein expression and collagen production. The LbL thin films containing EGF showed improved wound contraction (<9 days post excision). The co-delivery of TGF-β siRNA and EGF using LbL thin films resulted in accelerated wound healing and decreased collagen deposition. Furthermore, the LbL thin films with TGF-β siRNA and EGF combination showed greater reepithelialization. Taken together, we have successfully demonstrated the co-delivery of TGF-β siRNA and EGF peptide using LbL thin films to promote wound healing and decrease scar formation.read more
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Journal ArticleDOI
Macrophages with cellular backpacks for targeted drug delivery to the brain
Natalia L. Klyachko,Natalia L. Klyachko,Roberta Polak,Matthew J. Haney,Yuling Zhao,Reginaldo Jose Gomes Neto,Michael C. Hill,Alexander V. Kabanov,Alexander V. Kabanov,Robert E. Cohen,Michael F. Rubner,Elena V. Batrakova +11 more
TL;DR: A new method for drug delivery that utilizes living cells as vehicles for drug carriage across the blood brain barrier is reported, and catalase-loaded backpacks demonstrated potent therapeutic effects deactivating free radicals released by activated microglia in vitro.
Journal ArticleDOI
Upconversion Nanoparticle Powered Microneedle Patches for Transdermal Delivery of siRNA.
Min Wang,Min Wang,Yiyuan Han,Xiaojun Yu,Xiaojun Yu,Liangliang Liang,Hao Chang,David C. Yeo,Christian Wiraja,Mei Ling Wee,Linbo Liu,Xiaogang Liu,Chenjie Xu +12 more
TL;DR: A nanoparticle‐embedding MN system that contains a dissolvable hyaluronic acid (HA) matrix and mesoporous silica‐coated upconversion nanoparticles (UCNPs@mSiO2) shell is introduced that is used to deliver molecular beacons and siRNA targeting transforming growth factor‐beta type I receptor that is potentially used for abnormal scar treatment.
Journal ArticleDOI
Advancements and Challenges in Multidomain Multicargo Delivery Vehicles.
TL;DR: Success stories and challenges in the field of layer‐by‐layer, composite, self‐assembly, self-assembly, and core–shell technologies are discussed for the development of multidomain/multicargo delivery vehicles.
Journal ArticleDOI
Topical delivery of growth factors and metal/metal oxide nanoparticles to infected wounds by polymeric nanoparticles: an overview
Mehran Alavi,Mahendra Rai +1 more
TL;DR: Using various derivatives of chitosan as pH-responsive polymer with basic and acidic groups can be the best option to prepare controllable and sequential GF release but it warrants further extensive research to solve wound-healing problems.
Journal ArticleDOI
Polyelectrolyte Multilayers: An Overview on Fabrication, Properties, and Biomedical and Environmental Applications.
TL;DR: The main characteristics of polyelectrolyte multilayers, the fabrication methods currently used, and the factors influencing the layer-by-layer assembly of poly electrolytes are discussed in this paper.
References
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Factors Affecting Wound Healing
Shujuan Guo,Luisa A. DiPietro +1 more
TL;DR: The factors discussed include oxygenation, infection, age and sex hormones, stress, diabetes, obesity, medications, alcoholism, smoking, and nutrition, which may lead to therapeutics that improve wound healing and resolve impaired wounds.
Journal ArticleDOI
Preventing Foot Ulcers in Patients With Diabetes
TL;DR: Substantial evidence supports screening all patients with diabetes to identify those at risk for foot ulceration and recommending certain prophylactic interventions, including patient education, prescription footwear, intensive podiatric care, and evaluation for surgical interventions.
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Wound healing dressings and drug delivery systems: a review.
TL;DR: The requirement for formulations with improved properties for effective and accurate delivery of the required therapeutic agents and general formulation approaches towards achieving optimum physical properties and controlled delivery characteristics for an active wound healing dosage form are considered.
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Wound Repair and Regeneration
J.M. Reinke,H. Sorg +1 more
TL;DR: This review focuses on the healing processes of the skin and highlights the classical wound healing phases and the physiological endpoint of mammalian wound repair displays the formation of a scar, which is directly linked to the extent of the inflammatory process throughout wound healing.
Journal ArticleDOI
Determination of hydroxyproline.
TL;DR: A critical review is given of hydroxyproline analysis, finding a procedure which is faster and more sensitive than the former one and as reliable.