Limnochorda pilosa gen. nov., sp. nov., a moderately thermophilic, facultatively anaerobic, pleomorphic bacterium and proposal of Limnochordaceae fam. nov., Limnochordales ord. nov. and Limnochordia classis nov. in the phylum Firmicutes.
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TLDR
Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was distantly related to members of the family Symbiobacteriaceae and family XVII Incertae Sedis in the class Clostridia, and they formed a cluster separate from canonical species of the phylum Firmicutes.Abstract:
A novel facultatively anaerobic bacterium, strain HC45T, was isolated from sediment of a brackish meromictic lake in Japan, Lake Harutori. Cells were pleomorphic, and filamentous bodies were 5–100 μm in length. For growth, the optimum pH was 7.0 and the optimum temperature was 45–50 °C. The G+C content of the genomic DNA was 71 mol%. iso-C15 : 0 and anteiso-C15 : 0 were the major components in the cellular fatty acid profile. The predominant respiratory quinone was MK-7. Strain HC45T shared very low 16S rRNA gene sequence similarity with cultivated strains ( ≤ 85 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was distantly related to members of the family Symbiobacteriaceae and family XVII Incertae Sedis in the class Clostridia, and they formed a cluster separate from canonical species of the phylum Firmicutes. These results indicated that strain HC45T should not be placed in any existing class of the phylum Firmicutes. On the basis of phylogenetic and phenotypic characterization, Limnochorda pilosa gen. nov., sp. nov. is proposed with HC45T ( = NBRC 110152T = DSM 28787T) as the type strain, as the first representative of novel taxa, Limnochordales ord. nov., Limnochordaceae fam. nov. in Limnochordia classis. nov.read more
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Enhanced Growth and Activities of the Dominant Functional Microbiota of Chicken Manure Composts in the Presence of Maize Straw.
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Taxonomic hierarchy of the phylum Firmicutes and novel Firmicutes species originated from various environments in Korea
Chi Nam Seong,Joo Won Kang,Ji Hee Lee,So Yeon Seo,Jung Jae Woo,Chul Park,Kyung Sook Bae,Mi Sun Kim +7 more
TL;DR: Most Firmicutes species, belonging to the families Bacillaceae, Planococcaceae, and Staphylococc Families, isolated from Korean fermented foods and solar salterns were halophilic or halotolerant.
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Genome-wide analysis of the Firmicutes illuminates the diderm/monoderm transition.
Najwa Taib,Daniela Megrian,Daniela Megrian,Jerzy Witwinowski,Panagiotis S. Adam,Panagiotis S. Adam,Daniel Poppleton,Daniel Poppleton,Guillaume Borrel,Christophe Beloin,Simonetta Gribaldo +10 more
TL;DR: A third and deep branching diderm clade, the Limnochordia, is highlighted, strengthening the hypothesis of a diderm ancestor and the occurrence of independent transitions leading to the monoderm phenotype, and mapping the presence/absence of OM markers onto the tree of Bacteria shows the overwhelming presence of diderm phyla and the non-monophyly of monoderman ones.
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The microbial mechanisms of enhanced humification by inoculation with Phanerochaete chrysosporium and Trichoderma longibrachiatum during biogas residues composting.
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Microbial Life in the Deep Subsurface Aquifer Illuminated by Metagenomics.
TL;DR: The availability of buried organic matter of Mesozoic marine sediments, long-term recharge of the aquifer with meteoric waters and its spatial heterogeneity provided the conditions for the development of microbial communities, taxonomically and functionally more diverse than those found in oligotrophic underground ecosystems.
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