Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis
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TLDR
It is shown that LTP3 acts as a target of MYB96 to be involved in plant tolerance to freezing and drought stress, and consistently, transgenic plants overexpressing MyB96 exhibited enhanced freezing tolerance.Abstract:
Several lipid-transfer proteins were reported to modulate the plant response to biotic stress; however, whether lipid-transfer proteins are also involved in abiotic stress remains unknown. This study characterized the function of a lipid-transfer protein, LTP3, during freezing and drought stress. LTP3 was expressed ubiquitously and the LTP3 protein was localized to the cytoplasm. A biochemical study showed that LTP3 was able to bind to lipids. Overexpression of LTP3 resulted in constitutively enhanced freezing tolerance without affecting the expression of CBFs and their target COR genes. Further analyses showed that LTP3 was positively regulated by MYB96 via the direct binding to the LTP3 promoter; consistently, transgenic plants overexpressing MYB96 exhibited enhanced freezing tolerance. This study also found that the loss-of-function mutant ltp3 was sensitive to drought stress, whereas overexpressing plants were drought tolerant, phenotypes reminiscent of myb96 mutant plants and MYB96-overexpressing plants. Taken together, these results demonstrate that LTP3 acts as a target of MYB96 to be involved in plant tolerance to freezing and drought stress.read more
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References
More filters
Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Rapid determination of free proline for water-stress studies
TL;DR: In this article, a simple colorimetric determination of proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material was presented.
Journal ArticleDOI
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis.
TL;DR: The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments using molecular, cellular, biochemical, genetic, genomic and proteomic approaches to analyze the functions of diverse signaling pathways and cellular machineries.
Journal ArticleDOI
PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms
TL;DR: This review of recent advances in determining the nature and function of genes with roles in freezing tolerance and the mechanisms involved in low temperature gene regulation and signal transduction concludes that cold acclimation includes the expression of certain cold-induced genes that function to stabilize membranes against freeze-induced injury.
Journal ArticleDOI
Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis.
Qiang Liu,Mie Kasuga,Yoh Sakuma,Hiroshi Abe,Setsuko Miura,Kazuko Yamaguchi-Shinozaki,Kazuo Shinozaki +6 more
TL;DR: Overexpression of the DREB1A cDNA in transgenic Arabidopsis plants not only induced strong expression of the target genes under unstressed conditions but also caused dwarfed phenotypes in the transgenic plants, and revealed freezing and dehydration tolerance.