Journal ArticleDOI
Lipolytic enzymes of Trichophyton rubrum
S.K. Das,A.B. Banerjee +1 more
TLDR
Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.Abstract:
Trichophyton rubrum cells contain lipase, phospholipases A and B and acyl CoA lysolecithin acyl transferase activities. This dermatophyte excretes lipase and phospholipase A into the growth medium when cultivated in Sabouraud's broth. Extracellular lipase has optimum activity at pH 8.0 whereas the intracellular lipase is maximally active at pH 8.0 whereas the intracellular lipase is maximally active at pH 7.0. The optimum pH of phospholipase A and B activities which are localized in 15000 g sedimentable cell fragments are 7.0 and 6.0 respectively. Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.read more
Citations
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Book ChapterDOI
Potential for Penetration of Passive Barriers to Fungal Invasion in Humans
TL;DR: The focus of this chapter will be on the elaboration by fungi of enzymes that aid in the penetration of the host’s passive barriers.
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Purification of acyl CoA:1-acyl- sn -glycero-3-phosphorylcholine acyltransferase
V. C. Gavino,David W. Deamer +1 more
TL;DR: Acyl coenzyme A:1-acyl-sn-glycero-3-phosphorylcholine acyltransferase is capable of forming lipid bilayer vesicles from its soluble substrates lysophosphatidylcholine (LPC) and oleoyl CoA and is found to contain acyl transferase with a specific activity of 9–10 µmol/mg/min.
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Role of lipid metabolites in the signalling and activation of macrophage cells by lipopolysaccharide
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Secretion of five extracellular enzymes by strains of chromoblastomycosis agents
Thais Furtado de Souza,Maria Lúcia Scroferneker,Juliana Mônica da Costa,Mariana Carissimi,Valeriano Antonio Corbellini +4 more
TL;DR: The results indicate that only phospholipase production, induced by egg yolk substrate, was useful for the differentiation of the taxonomically related species studied, based on their enzymatic profile.
Journal ArticleDOI
Effect of undecanoic acid on phospholipid metabolism in Trichophyton rubrum
S.K. Das,A.B. Banerjee +1 more
TL;DR: At its minimum inhibitory concentration, undecanoic acid (UDA) inhibits biosynthesis of phosphatidyl serine, phosph atidyl ethanolamine and polyphosphoinositol but does not inhibit the synthesis of phosphatin, so it may be partially responsible for its toxic action on Trichophyton rubrum.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
TL;DR: In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.
Journal ArticleDOI
Phospholipids of Trichophyton rubrum.
S.K. Das,A.B. Banerjee +1 more
TL;DR: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatids inositols, and the relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
Journal ArticleDOI
Lipolytic activity of dermatophytes.
G. Nobre,Maria Paula Viegas +1 more
TL;DR: Among subcultured strains, it was found a decreased lipolytic activity in Microsporum species and an increased lipolyic activity in Epidermophyton and someTrichophyton species.
Journal ArticleDOI
Phospholipid turnover in Trichophyton rubrum
S.K. Das,A.B. Banerjee +1 more
TL;DR: Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer, and their conversions to phosphatidyl choline and phosph atidyl inositol are suspected.