Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
Warren R. Zipfel,Rebecca M. Williams,R. H. Christie,Alexander Yu. Nikitin,Bradley T. Hyman,Watt W. Webb +5 more
TLDR
Applications involving a range of intrinsic molecules and molecular assemblies that enable direct visualization of tissue morphology, cell metabolism, and disease states such as Alzheimer's disease and cancer are compiled and demonstrated.Abstract:
Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, indoleamines, and collagen provides crucial information for physiology and pathology. The efficient application of multiphoton microscopy to intrinsic imaging requires knowledge of the nonlinear optical properties of specific cell and tissue components. Here we compile and demonstrate applications involving a range of intrinsic molecules and molecular assemblies that enable direct visualization of tissue morphology, cell metabolism, and disease states such as Alzheimer's disease and cancer.read more
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Nonlinear magic: multiphoton microscopy in the biosciences
TL;DR: Multiphoton microscopy has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals and its use is now increasing exponentially.
Journal ArticleDOI
Fluorescence Lifetime Measurements and Biological Imaging
TL;DR: The lifetime of a photophysical process is the time required by a population of N electronically excited molecules to be reduced by a factor of e via the loss of energy through fluorescence and other non-radiative processes and the average length of time τ is called the mean lifetime, or simply lifetime.
Journal ArticleDOI
Collagen reorganization at the tumor-stromal interface facilitates local invasion
Paolo P. Provenzano,Kevin W. Eliceiri,Jay M. Campbell,David R. Inman,John G. White,Patricia J. Keely +5 more
TL;DR: Three tumor-associated collagen signatures (TACS) are observed and defined that provide novel markers to locate and characterize tumors and should provide indications that a tumor is, or could become, invasive, and may serve as part of a strategy to help identify and characterize breast tumors in animal and human tissues.
Journal ArticleDOI
Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms.
TL;DR: Recent studies of the three-dimensional in vivo structures of well-ordered protein assemblies, such as collagen, microtubules and muscle myosin, are beginning to establish SHIM as a nondestructive imaging modality that holds promise for both basic research and clinical pathology.
Journal ArticleDOI
Collagen density promotes mammary tumor initiation and progression
Paolo P. Provenzano,David R. Inman,Kevin W. Eliceiri,Justin G Knittel,Long Yan,Curtis Rueden,John G. White,Patricia J. Keely +7 more
TL;DR: This study provides the first data causally linking increased stromal collagen to mammary tumor formation and metastasis, and demonstrates that fundamental differences arise and persist in epithelial tumor cells that progressed within collagen-dense microenvironments.
References
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Principles of Optics
TL;DR: In this paper, the authors discuss various topics about optics, such as geometrical theories, image forming instruments, and optics of metals and crystals, including interference, interferometers, and diffraction.
Principles of Optics
TL;DR: In this article, the authors discuss various topics about optics, such as geometrical theories, image forming instruments, and optics of metals and crystals, including interference, interferometers, and diffraction.
Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
Quantitative optical spectroscopy for tissue diagnosis
TL;DR: This review describes optical interactions pursued for biomedical applications (fluorescence, fluorescence lifetime, phosphorescence, and Raman from cells, cultures, and tissues) and provides a descriptive framework for light interaction based upon tissue absorption and scattering properties.
Journal ArticleDOI
Expression of the neu protooncogene in the mammary epithelium of transgenic mice induces metastatic disease
Chantale T. Guy,Marc A. Webster,Michael Schaller,Thomas J. Parsons,Robert D. Cardiff,William J. Muller +5 more
TL;DR: Overexpression of the unactivated neu protein can induce metastatic disease after long latency and is associated with elevated neu intrinsic tyrosine kinase activity and the de novo tyrosinesine phosphorylation of several cellular proteins.