Maize regulatory gene opaque-2 encodes a protein with a "leucine-zipper" motif that binds to zein DNA
TLDR
The opaque-2 locus (o2) in maize regulates the expression of many members of the zein multigene family of storage proteins as mentioned in this paper, and the presumptive amino acid sequence of the protein (O2) specified by the O2 cDNA contains a "leucine-zipper" domain characteristic of some mammalian and fungal transcription activation factors.Abstract:
The opaque-2 locus (o2) in maize regulates the expression of many members of the zein multigene family of storage proteins. cDNA clones for a wild-type allele of the (o2) locus (O2) were isolated from a maize endosperm cDNA library and sequenced. We found a 258-nucleotide 5' leader sequence containing three short open reading frames followed by a sequence specifying a protein of 437 amino acids. The presumptive amino acid sequence of the protein (O2) specified by the O2 cDNA contains a "leucine-zipper" domain characteristic of some mammalian and fungal transcription activation factors. lacZ-O2 fusion constructs, using nearly the entire coding region of O2 or only a fragment specifying the leucine-zipper domain, were expressed in Escherichia coli. In an in vitro binding assay, the beta-galactosidase-O2 fusion proteins bound to two specific regions on the 5' side of the coding sequence in a zein genomic clone. This suggests that the O2 protein affects zein transcription through direct interaction with one or more zein promoter elements.read more
Citations
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A plant leucine zipper protein that recognizes an abscisic acid response element
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AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes
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