Maternal nutrient restriction in early pregnancy programs hepatic mRNA expression of growth-related genes and liver size in adult male sheep.
Melanie A. Hyatt,G. S. Gopalakrishnan,J. Bispham,Sheridan Gentili,Isabella Caroline McMillen,Stewart M. Rhind,M. T. Rae,Carol Kyle,A. N. Brooks,C Jones,Helen Budge,David Walker,Terence Stephenson,Michael E. Symonds +13 more
Reads0
Chats0
TLDR
Maternal nutrient restriction in early gestation results in adult offspring with smaller livers, which may be mediated by alterations in both hepatic mitogenic and apoptotic factors.Abstract:
The liver is a major metabolic and endocrine organ of critical importance in the regulation of growth and metabolism. Its function is determined by a complex interaction of nutritionally regulated counter-regulatory hormones. The extent to which hepatic endocrine sensitivity can be programed in utero and whether the resultant adaptations persist into adulthood is unknown and was therefore the subject of this study. Young adult male sheep born to mothers that were fed either a control diet (i.e.100% of total live weight-maintenance requirements) throughout gestation or 50% of that intake (i.e. nutrient restricted (NR)) from 0 to 95 days gestation and thereafter 100% of requirements (taking into account increasing fetal mass) were entered into the study. All mothers gave birth normally at term, the singleton offspring were weaned at 16 weeks, and then reared at pasture until 3 years of age when their livers were sampled. NR offspring were of similar birth and body weights at 3 years of age when they had disproportionately smaller livers than controls. The abundance of mRNA for GH, prolactin, and IGF-II receptors, plus hepatocyte growth factor and suppressor of cytokine signaling-3 were all lower in livers of NR offspring. In contrast, the abundance of the mitochondrial protein voltage-dependent anion channel and the pro-apoptotic factor Bax were up regulated relative to controls. In conclusion, maternal nutrient restriction in early gestation results in adult offspring with smaller livers. This may be mediated by alterations in both hepatic mitogenic and apoptotic factors.read more
Citations
More filters
Journal Article
Adverse effects of nutritional programming during prenatal and early postnatal life, some aspects of regulation and potential prevention and treatments.
TL;DR: Early infancy may provide an opportunity for intervention aimed at reducing later disease risk and the development of new, rational, and effective preventive and/ or therapeutic options before and/or after birth are suggested.
Journal ArticleDOI
A review of fundamental principles for animal models of DOHaD research: An Australian perspective
Hayley Dickinson,Timothy J. M. Moss,Kathryn L. Gatford,Karen M. Moritz,Lisa K. Akison,Tod Fullston,Deanne H. Hryciw,Christopher A. Maloney,Margaret J. Morris,Amy L. Wooldridge,John E. Schjenken,Sarah A. Robertson,Brendan J. Waddell,Peter J. Mark,Caitlin S. Wyrwoll,Stacey J. Ellery,Kent L. Thornburg,Beverly S. Muhlhausler,Janna L. Morrison +18 more
TL;DR: This review summarizes the contributions of animal research to the understanding of DOHaD, and makes recommendations for the design and conduct of animal experiments to maximize relevance, reproducibility and translation of knowledge into improving health and well-being.
Journal ArticleDOI
Maternal undernutrition and the ovine acute phase response to vaccination
Peter David Eckersall,Fraser P Lawson,Carol Kyle,Mary Waterston,Laura Bence,Michael J. Stear,Stewart M. Rhind +6 more
TL;DR: Decreased SAA concentrations, post-vaccination, in lambs born to ewes on the HL diet shows that maternal undernutrition prior to parturition affects the innate immune system of the offspring.
Journal ArticleDOI
Nutrient intake in the bovine during early and mid-gestation causes sex-specific changes in progeny plasma IGF-I, liveweight, height and carcass traits
TL;DR: Heifer nutrient intake during the first and second trimesters causes persistent and sex-specific programming of progeny plasma IGF-I, postnatal liveweight and carcass weight.
Journal ArticleDOI
Hypertension and impaired renal function accompany juvenile obesity: the effect of prenatal diet.
P.J. Williams,Lesia O. Kurlak,Alan C. Perkins,Helen Budge,T Stephenson,Duane H. Keisler,Michael E. Symonds,David S. Gardner +7 more
TL;DR: In this paper, the effects of prenatal nutrient restriction and juvenile obesity in sheep were examined and it was found that juvenile obesity led to chronic hyperleptinemia and reduced renal function as assessed by nuclear scintigraphy.
References
More filters
Journal ArticleDOI
Differential expression of the growth hormone receptor and growth hormone-binding protein in epithelia and stroma of the mouse mammary gland at various physiological stages.
TL;DR: GHR and GHBP mRNAs and proteins are expressed in both the epithelium and the stroma of mammary glands of virgin, pregnant, and lactating mice and indicate that the actions of GH in the mammary gland are both direct through its binding tothe epithelia, and indirect by binding to theStroma and stimulation of IGF-I production which, in turn, affects mammary epithelial development.
Journal ArticleDOI
Ontogeny and nutritional manipulation of the hepatic prolactin-growth hormone-insulin-like growth factor axis in the ovine fetus and in neonate and juvenile sheep.
TL;DR: Maternal undernutrition during the various stages of gestation reprogrammed the PRL–GH–IGF axis and endocrine sensitivity, and nutritional regulation of cytokine receptors may contribute to altered liver function following the onset of GH-dependent growth.
Journal ArticleDOI
Maternal nutrient restriction during early to mid gestation alters the relationship between insulin-like growth factor I and bodyweight at term in fetal sheep
L. Heasman,John M. Brameld,Alison Mostyn,Helen Budge,J. M. Dawson,Peter J. Buttery,Terence Stephenson,Michael E. Symonds +7 more
TL;DR: There is a loss of the relationship between fetal weight and plasma IGF-I concentration observed in fetuses whose mothers are fed adequately throughout gestation, and increasing maternal nutrition in later gestation after a prolonged period of nutrient restriction may stimulate fetal nutrient supply such that fetal growth is enhanced without an increase in plasma IGF -I.
Journal ArticleDOI
Hepatic prolactin receptor gene expression increases in the sheep fetus before birth and after cortisol infusion.
TL;DR: Fetal PRL may play a role in the growth and maturation of the fetal liver which occurs before birth and physiological increases in fetal cortisol stimulate PRLR1 and PRLR2 expression in the liver of the sheep fetus.
Journal ArticleDOI
Nutritional regulation of IGF-II, but not IGF-I, is age dependent in sheep
TL;DR: The response of plasma IGF-II to fasting suggests that this peptide has functions in mediating nutritional stress which depend on the age of the animal, and also that the role of IGF -II may differ from that of IGF-I in adults.