Journal ArticleDOI
Mediation of glycosylated and partially-deglycosylated glucose oxidase of Aspergillus niger by a ferrocene-derivatised detergent
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TLDR
A ferrocene-derivatised detergent, (11-ferrocenylundecyl) trimethylammonium bromide (FTMAB), when oxidised to the corresponding ferricinium ion, was found by electrochemical studies to be an effective electron acceptor for reduced glucose oxidation of Aspergillus niger and thus acts as a electron-transfer mediator between glucose oxidase and a working electrode held at a potential sufficiently positive to reoxAbout:
This article is published in Biochimica et Biophysica Acta.The article was published on 1992-01-30. It has received 29 citations till now. The article focuses on the topics: Aspergillus niger.read more
Citations
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Electrochemical Principles for Active Control of Liquids on Submillimeter Scales
Benedict S. Gallardo,Vinay Gupta,Franklin D. Eagerton,Lana I. Jong,Vincent S. J. Craig,Rahul R. Shah,Nicholas L. Abbott +6 more
TL;DR: Electrochemical methods were combined with redox-active surfactants to actively control the motions and positions of aqueous and organic liquids on millimeter and smaller scales to guide droplets of organic liquids through simple fluidic networks.
Journal ArticleDOI
Towards mediator design: Characterization of tris-(4,4′-substituted-2,2′-bipyridine) complexes of iron(II), ruthenium(II) and osmium(II) as mediators for glucose oxidase of Aspergillus niger and other redox proteins
TL;DR: A range of tris-(4,4′-substituted-2,2′-bipyridine) complexes have been synthesized and characterized electrochemically with respect to their ability to act as electron transfer mediators for redox enzymes, notably glucose oxidase (GOD, EC 1.1.4) of Aspergillus niger as discussed by the authors.
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Deglycosylation of Glucose Oxidase for Direct and Efficient Glucose Electrooxidation on a Glassy Carbon Electrode
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Structural and Kinetic Properties of Nonglycosylated Recombinant Penicillium amagasakiense Glucose Oxidase Expressed in Escherichia coli
TL;DR: The reactivated recombinant GOX was purified to homogeneity by mild acidification and anion-exchange chromatography and exhibits kinetics of glucose oxidation similar to those of, but lower pH and thermal stabilities than, native GOX from P. amagasakiense.
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Engineering glucose oxidase for bioelectrochemical applications.
TL;DR: The recent progress in glucose oxidase reengineering is presented and the need to develop new screening methods involving electrochemical probing, essential to develop the next generation of glucose sensors; specific to glucose, O2 independent, biocompatible and stable over 2 weeks is highlighted.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Theory of Stationary Electrode Polarography. Single Scan and Cyclic Methods Applied to Reversible, Irreversible, and Kinetic Systems.
R. S. Nicholson,Irving. Shain +1 more
TL;DR: In this paper, the integral equations obtained from the boundary value problems were solved and extensive data were calculated which permit construction of stationary electrode polarograms from theory, making it possible to develop diagnostic criteria so that unknown systems can be characterized by studying the variation of peak current, half-peak potential, or ratio of anodic to cathodic peak currents as a function of rate of voltage scan.
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Ferrocene-mediated enzyme electrode for amperometric determination of glucose.
Anthony E. G. Cass,Graham Davis,Francis Gd,Hugh Allen Oliver Hill,W.J. Aston,I.J. Higgins,E. V. Plotkin,Scott Ld,Anthony Turner +8 more
TL;DR: Type III adenosine deaminase would be the best choice for the construction of an immobilized enzyme electrode both from the point of view of apparent Km and Vmax values and from the less pronounced product inhibition effect on the type III enzyme compared to the Type V enzyme.
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Cross-linked redox gels containing glucose oxidase for amperometric biosensor applications.
Brian A. Gregg,Adam Heller +1 more
TL;DR: Two materials for amperometric biosensors based on a cross-linkable poly(vinylpyridine) complex of [Os-(bpy)2Cl]+2+ that communicates electrically with flavin adenine dinucleotide redox centers of enzymes such as glucose oxidase are described.