Journal ArticleDOI
Methanotrophic Community Detected by DNA-SIP at Bertioga’s Mangrove Area, Southeast Brazil
Débora do Carmo Linhares,Flávia Talarico Saia,Rubens Tadeu Delgado Duarte,Cristina Rossi Nakayama,Itamar Soares de Melo,Vivian Helena Pellizari +5 more
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TLDR
In this paper, the functional and taxonomic diversity of methanotrophic bacteria present in the anthropogenic impacted sediments from Bertioga´s mangrove (SP, Brazil).Abstract:
Methanotrophic bacteria can use methane as sole carbon and energy source. Its importance in the environment is related to the mitigation of methane emissions from soil and water to the atmosphere. Brazilian mangroves are highly productive, have potential to methane production, and it is inferred that methanotrophic community is of great importance for this ecosystem. The scope of this study was to investigate the functional and taxonomic diversity of methanotrophic bacteria present in the anthropogenic impacted sediments from Bertioga´s mangrove (SP, Brazil). Sediment sample was cultivated with methane and the microbiota actively involved in methane oxidation was identified by DNA-based stable isotope probing (DNA-SIP) using methane as a labeled substrate. After 4 days (96 h) of incubation and consumption of 0.7 mmol of methane, the most active microorganisms were related to methanotrophs Methylomonas and Methylobacter as well as to methylotrophic Methylotenera, indicating a possible association of these bacterial groups within a methane-derived food chain in the Bertioga mangrove. The abundance of genera Methylomonas, able to couple methane oxidation to nitrate reduction, may indicate that under low dissolved oxygen tensions, some aerobic methanotrophs could shift to intraerobic methane oxidation to avoid oxygen starvation.read more
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Acetate excretion by a methanotroph, Methylocaldum marinum S8, under aerobic conditions.
Mio Takeuchi,Hideyoshi Yoshioka +1 more
TL;DR: In this paper, the effects of oxygen concentrations on growth of and acetate excretion by Methylocaldum marinum S8 in pure culture and the coculture with M. caenitepidi Gela4T were examined.
Journal ArticleDOI
In-situ active Bisphenol A-degrading microorganisms in mangrove sediments.
TL;DR: In this article, DNA-based stable isotope probing in combination with high-throughput sequencing was adopted to pinpoint the microbes actually involved in BPA metabolism in mangrove sediments.
Journal ArticleDOI
Enrichment of Methylosinus-dominant consortia from mangroves for polyhydroxybutyrate (PHB) production
Letícia Oliveira Bispo Cardoso,Bruno Karolski,Louise Hase Gracioso,B. Borrego,Cláudio Augusto Oller do Nascimento,Elen Aquino Perpetuo +5 more
TL;DR: In this article , a consortium of Methylosinus -dominant consortia was found to be capable of producing polyhydroxybutyrate (PHB), a biodegradable polymer produced by methanotrophic bacteria that could substitute plastics and sequester GHGs from the atmosphere.
Journal ArticleDOI
In-situ active Bisphenol A-degrading microorganisms in mangrove sediments
TL;DR: In this article , DNA-based stable isotope probing in combination with high-throughput sequencing was adopted to pinpoint the microbes actually involved in BPA metabolism in mangrove sediments.
Journal ArticleDOI
Use of methanotrophically activated biochar in novel biogeochemical cover system for carbon sequestration: Microbial characterization.
TL;DR: In this article , column tests were performed to simulate the newly proposed biogeochemical cover systems, which incorporate biochar-amended soil for CH4 oxidation and basic oxygen furnace (BOF) slag for CO2 and H2S mitigation.
References
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Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
Patrick D. Schloss,Patrick D. Schloss,Sarah L. Westcott,Sarah L. Westcott,Thomas Ryabin,Justine R. Hall,Martin Hartmann,Emily B. Hollister,Ryan A. Lesniewski,Brian B. Oakley,Donovan H. Parks,Courtney J. Robinson,Jason W. Sahl,Blaz Stres,Gerhard G. Thallinger,David J. Van Horn,Carolyn F. Weber +16 more
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ARB: a software environment for sequence data
Wolfgang Ludwig,Oliver Strunk,Ralf Westram,Lothar Richter,Harald Meier,Yadhukumar,Arno Buchner,Tina Lai,Susanne Steppi,Gangolf Jobb,Wolfram Förster,Igor Brettske,Stefan Gerber,Anton W. Ginhart,Oliver Gross,Silke Grumann,Stefan Hermann,Ralf Jost,Andreas König,Thomas Liss,Ralph Lüßmann,Michael May,Björn Nonhoff,Boris Reichel,Robert Strehlow,Alexandros Stamatakis,Norbert Stuckmann,Alexander Vilbig,Michael Lenke,Thomas Ludwig,Arndt Bode,Karl-Heinz Schleifer +31 more
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