Journal ArticleDOI
Selection of associated heterotrophs by methane-oxidizing bacteria at different copper concentrations
TLDR
Interpretative tools based on denaturing gradient gel electrophoresis allowed to evaluate the influence of copper—an important enzymatic regulator for MOB—on the activity and composition of the bacterial community, indicating that the MOB selected for certain heterotrophs, possibly fulfilling vital processes such as removal of toxic compounds.Abstract:
Due to the increasing atmospheric concentration of the greenhouse gas methane, more knowledge is needed on the management of methanotrophic communities. While most studies have focused on the characteristics of the methane-oxidizing bacteria (MOB), less is known about their interactions with the associated heterotrophs. Interpretative tools based on denaturing gradient gel electrophoresis allowed to evaluate the influence of copper—an important enzymatic regulator for MOB—on the activity and composition of the bacterial community. Over 30 days, enrichments with 0.1, 1.0 and 10 μM Cu2+ respectively, showed comparable methane oxidation activities. The different copper concentrations did not create major shifts in the methanotrophic communities, as a Methylomonas sp. was able to establish dominance at all different copper concentrations by switching between both known methane monooxygenases. The associated heterotrophic communities showed continuous shifts, but over time all cultures evolved to a comparable composition, independent of the copper concentration. This indicates that the MOB selected for certain heterotrophs, possibly fulfilling vital processes such as removal of toxic compounds. The presence of a large heterotrophic food web indirectly depending on methane as sole carbon and energy source was confirmed by a clone library wherein MOB only formed a minority of the identified species.read more
Citations
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Journal ArticleDOI
A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcaceae and the Methylophilaceae
David A. C. Beck,Marina G. Kalyuzhnaya,Stephanie Malfatti,Stephanie Malfatti,Susannah G. Tringe,Tijana Glavina del Rio,Natalia Ivanova,Mary E. Lidstrom,Ludmila Chistoserdova +8 more
TL;DR: The relevant abundances of the M methylococcaceae and the Methylophilaceae and their coordinated response to methane and nitrate suggest that these species may be engaged in cooperative behavior, the nature of which remains unknown.
Journal ArticleDOI
Methane-fed microbial microcosms show differential community dynamics and pinpoint taxa involved in communal response.
Igor Y. Oshkin,Igor Y. Oshkin,David A. C. Beck,Andrew Lamb,Veronika Tchesnokova,Gabrielle Benuska,Tami L. McTaggart,Marina G. Kalyuzhnaya,Svetlana N. Dedysh,Mary E. Lidstrom,Ludmila Chistoserdova +10 more
TL;DR: The results support prior observations from metagenomics on distribution of carbon from methane among diverse bacterial populations and further suggest that communities are likely responsible for methane cycling, rather than a single type of microbe.
Journal ArticleDOI
Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation
TL;DR: The data suggest that oxygen availability is at least one major factor determining specific partnerships in methane oxidation, and suggest that speciation within Methylococcaceae and Methylophilaceae may be driven by niche adaptation tailored toward specific placements within the oxygen gradient.
Journal ArticleDOI
Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity.
Frederiek-Maarten Kerckhof,Emilie Courtens,Annelies Geirnaert,Sven Hoefman,Adrian Ho,Ramiro Vilchez-Vargas,Dietmar H. Pieper,Ruy Jauregui,Siegfried E. Vlaeminck,Tom Van de Wiele,Peter Vandamme,Kim Heylen,Nico Boon +12 more
TL;DR: A cryopreservation protocol is evaluated that succeeded in preserving both community structure and functionality of value-added microbiomes and will allow individual laboratories and culture collections to boost the use of microbiomes in biotechnological applications.
Journal ArticleDOI
Exploration and prediction of interactions between methanotrophs and heterotrophs.
Michiel Stock,Sven Hoefman,Frederiek-Maarten Kerckhof,Nico Boon,Paul De Vos,Bernard De Baets,Kim Heylen,Willem Waegeman +7 more
TL;DR: Modelling-based tools were shown to hold great promise in reducing the amount of data that needs to be generated when conducting large co-cultivation studies, suggesting strain-dependent methanotroph-heterotroph complementarity.
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Journal ArticleDOI
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Journal ArticleDOI
Methanotrophs and copper
TL;DR: The current state of knowledge of the phylogeny, environmental distribution, and potential applications of methanotrophs for regional and global issues are summarized, as well as the role of Cu in regulating gene expression and proteome in these cells, its effects on enzymatic and whole-cell activity, and the novel Cu uptake system used by methanOTrophs are summarized.
Journal ArticleDOI
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Journal ArticleDOI
Molecular Characterization of Functional and Phylogenetic Genes from Natural Populations of Methanotrophs in Lake Sediments
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