Journal ArticleDOI
Method of obtaining optical sectioning by using structured light in a conventional microscope
TLDR
A simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object and processing images that are substantially similar to those obtained with confocal microscopes is described.Abstract:
We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid are processed in real time to produce optically sectioned images that are substantially similar to those obtained with confocal microscopes.read more
Citations
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Journal ArticleDOI
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution
TL;DR: Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples, and a recently proposed method in which the nonlinearity arises from saturation of the excited state is experimentally demonstrated.
Journal ArticleDOI
Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy.
TL;DR: This work developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development to derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event.
Journal ArticleDOI
Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination
Mats G. L. Gustafsson,Lin Shao,Peter M. Carlton,C. J. Rachel Wang,Inna N. Golubovskaya,W. Zacheus Cande,David A. Agard,David A. Agard,John W. Sedat +8 more
TL;DR: This work describes how spatially structured illumination microscopy can be applied in three dimensions to double the axial as well as the lateral resolution, with true optical sectioning, and has produced the first light microscopy images of the synaptonemal complex in which the lateral elements are clearly resolved.
Journal ArticleDOI
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
Thomas A. Planchon,Liang Gao,Daniel E. Milkie,Michael W. Davidson,James A. Galbraith,Catherine G. Galbraith,Eric Betzig +6 more
TL;DR: Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.
References
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Journal ArticleDOI
Theory and practice of scanning optical microscopy
Tony Wilson,Colin J. R. Sheppard +1 more
Journal ArticleDOI
Properties of a Defocused Optical System
TL;DR: In this article, the exact diffraction optical transfer function (OTF) and point spread function (PSF) are compared to the geometrical OTF and PSF, respectively, for both small and large amounts of defocusing.
Patent
Optical sensor for imaging an object
TL;DR: In this article, a sensor consisting of a structured light source (5, 6, 7) which is adjustable so as to interchange the positions of contrasting areas of the pattern it provides, a detector (1) which comprises an array of detector elements having dimensions matched to the pattern produced by the light source, an optical system (2, 8) for projecting a primary image of a light source pattern onto an object (3) that is to be sensed and for forming a secondary image on the detector(1) of the primary image thus formed on the object, positioning means (
Journal ArticleDOI
The axial response of confocal microscopes with high numerical aperture objective lenses
Tony Wilson,R Jus̆kaitis +1 more
TL;DR: The axial resolution of confocal optical systems employing high numerical aperture objective lenses for the cases when the specimen refractive index is both higher and lower than that of the immersion medium was considered in this paper.
Proceedings ArticleDOI
Simultaneous Amplitude Modulation Contrast (SAMC) A New Optical Imaging Technique
TL;DR: In this paper, a new kind of contrast for an optical microscope is presented, which can be realized by first imaging a rotating grating onto the object and then imaging the grating together with the object to a second rotated grating positioned in an intermediate image plane.