Journal ArticleDOI
MicroRNA-29b Inhibits Endometrial Fibrosis by Regulating the Sp1-TGF-β1/Smad-CTGF Axis in a Rat Model
Reads0
Chats0
TLDR
Agomir-29b may act as a novel and effective therapeutic agent against IUAs and inhibited endometrial fibrosis via blockade of the Sp1-TGF-β1/Smad-CTGF pathway, in conclusion.Abstract:
Intrauterine adhesions (IUAs), which are characterized by endometrial fibrosis, increase the risk of secondary infertility and recurrent miscarriage. MicroRNA-29 (miR-29) is a potent inhibitor of TGF-β1/Smad signaling. In this study, we investigated the therapeutic potential of agomir-29b, an miR-29b mimic, in endometrial fibrosis induced by dual injury (uterine curettage and lipopolysaccharide treatment) in a rat model of IUA and explored the underlying mechanism. We found that injured rats developed endometrial fibrosis characterized by increased COL1A1 and α-smooth muscle actin expression and decreased E-cadherin expression, associated with a loss of miR-29b. Overexpression of miR-29b before injury prevented endometrial fibrosis including collagen accumulation and epithelial-mesenchymal transition. Delay of agomir-29b treatment until endometrial fibrosis was established on day 4 also halted the progression of disease. Further experiments indicated that miR-29b inhibited endometrial fibrosis via blockade of the Sp1-TGF-β1/Smad-CTGF pathway. In conclusion, agomir-29b may act as a novel and effective therapeutic agent against IUAs.read more
Citations
More filters
Journal ArticleDOI
Exosomes derived from mesenchymal stem cells reverse EMT via TGF-β1/Smad pathway and promote repair of damaged endometrium
TL;DR: BMSC-derived Exo is involved in the repair of injured endometrium, with similar effect to that of BMSC, and can reverse EMT in rabbit EECs induced by TGF-β1.
Journal ArticleDOI
LncRNA PVT1 regulates atrial fibrosis via miR-128-3p-SP1-TGF-β1-Smad axis in atrial fibrillation
TL;DR: PVT1 was increased in atrial muscle tissues from AF patients and positively with collagen I and collagen III, and Mechanically, PVT1 acted as a sponge for miR-128-3p to facilitate Sp1 expression, thereby activating the TGF-β1/Smad signaling pathway.
Journal ArticleDOI
Long noncoding RNA H19 accelerates tenogenic differentiation and promotes tendon healing through targeting miR-29b-3p and activating TGF-β1 signaling.
Ying-fei Lu,Yang Liu,Weiming Fu,Jia Xu,Jia Xu,Bin Wang,Yuxin Sun,Tianyi Wu,Liangliang Xu,Kai-Ming Chan,Jinfang Zhang,Gang Li +11 more
TL;DR: This study found that lncRNA H19 stimulated tenogenesis of human tendon‐derived stem cells and promotes tenogenic differentiation both in vitro and in vivo by targeting miR‐29b‐3p and activating TGF‐β1 signaling.
Journal ArticleDOI
Platelet-rich plasma improves therapeutic effects of menstrual blood-derived stromal cells in rat model of intrauterine adhesion
TL;DR: MenSCs could effectively improve uterine proliferation, markedly accelerate endometrial damage repairment and promote fertility restoration in IUA rats, suggesting a paracrine restorative effect and Hippo signaling pathway stimulation.
Journal ArticleDOI
Early-Life Exposure to Lead (Pb) Alters the Expression of microRNA that Target Proteins Associated with Alzheimer's Disease.
TL;DR: It is shown that exposure to the heavy metal Pb in early life has a significant impact on the short- and long-term expression of miRNA that target epigenetic mediators and neurotoxic proteins.
References
More filters
Journal ArticleDOI
MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
TL;DR: Although they escaped notice until relatively recently, miRNAs comprise one of the more abundant classes of gene regulatory molecules in multicellular organisms and likely influence the output of many protein-coding genes.
Journal ArticleDOI
Dysregulation of microRNAs after myocardial infarction reveals a role of miR-29 in cardiac fibrosis
Eva van Rooij,Lillian B. Sutherland,Jeffrey E. Thatcher,J. Michael DiMaio,R. Haris Naseem,William S. Marshall,Joseph A. Hill,Eric N. Olson +7 more
TL;DR: It is concluded that miR-29 acts as a regulator of cardiac fibrosis and represents a potential therapeutic target for tissue fibrosis in general.
Journal ArticleDOI
New insights into TGF-beta-Smad signalling.
Peter ten Dijke,Caroline S. Hill +1 more
TL;DR: New insights are provided into the specificity determinants of TGF-β–Smad signalling, including combinatorial ligand–receptor associations, selective interactions between the Smads and other pathway components that are mediated through defined binding motifs, and the differential regulation of duration and intensity of signalling.
Journal ArticleDOI
NF-κB–YY1–miR-29 Regulatory Circuitry in Skeletal Myogenesis and Rhabdomyosarcoma
Huating Wang,Ramiro Garzon,Hao Sun,Katherine J. Ladner,Ravi K. Singh,Jason M. Dahlman,Alfred S. L. Cheng,Brett Hall,Stephen J. Qualman,Dawn S. Chandler,Carlo M. Croce,Denis C. Guttridge +11 more
TL;DR: Reconstitution of miR-29 in RMS in mice inhibits tumor growth and stimulates differentiation, suggesting that mi R-29 acts as a tumor suppressor through its promyogenic function.
Journal ArticleDOI
Asherman syndrome—one century later
TL;DR: The management of moderate to severe disease still poses a challenge, and the prognosis of severe disease remains poor, so close antenatal surveillance and monitoring are necessary for women who conceive after treatment.