Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions
Susanne A. I. Seidel,Patricia M. Dijkman,Wendy A. Lea,Geert van den Bogaart,Moran Jerabek-Willemsen,Ana Lazic,Jeremiah S. Joseph,Prakash Srinivasan,Philipp Baaske,Anton Simeonov,Ilia Katritch,Fernando A. Melo,John E. Ladbury,Gideon Schreiber,Anthony Watts,Dieter Braun,Stefan Duhr +16 more
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TLDR
Flexibility in assay design qualifies MST for analysis of biomolecular interactions in complex experimental settings, which is demonstrated by addressing typically challenging types of binding events from various fields of life science.About:
This article is published in Methods.The article was published on 2013-03-01 and is currently open access. It has received 508 citations till now. The article focuses on the topics: Microscale thermophoresis & Thermophoresis.read more
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Multiphasic effect of vinyl pyrrolidone polymers on amyloidogenesis, from macromolecular crowding to inhibition
TL;DR: It is demonstrated that high molecular mass PVP360 promotes amyloidogenesis due to volume exclusion and increase in effective amyloidsogenic monomer concentration, like other crowders, but without the confounding effects of viscosity and surface activity.
Book ChapterDOI
Step-By-Step In Vitro Mutagenesis: Lessons From Fucose-Binding Lectin PA-IIL
TL;DR: A site-directed mutagenesis method used for investigating the sugar binding pattern of the PA-IIL lectin from the pathogenic bacterium Pseudomonas aeruginosa is described, and several basic methods for characterizing the mutants are discussed.
Dissertation
Ionic thermophoresis and its application in living cells
TL;DR: The first result of this thesis is that the phenomenon thermophoresis consists of different additive contributions, which bridge the gap between local thermodynamic equilibrium approaches and non-equilibrium theories.
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Chlorhexidine as a Keap1-Nrf2 inhibitor: a new target for an old drug for Parkinson's disease therapy
Sruthi Unni,Prashant Deshmukh,Gopinatha Krishnappa,M.M. Srinivas Bharath,Balasundaram Padmanabhan +4 more
TL;DR: Chlorhexidine, an antimicrobial agent, is widely used as a mouthwash, skin cleanser, and intervening bacterial infection during childbirth, and the chemical scaffold of chlorhexidine is a potential lead to develop new chemical libraries with drug-like properties for treating PD.
Journal ArticleDOI
Biophysical methods for the study of protein interactions.
Peter Schuck,Huaying Zhao +1 more
TL;DR: The goal of the present Special Issue is to represent this rapid development on many levels, in a periscope-like fashion through detailed introductions to select current developments by leaders in the field.
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