Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions
Susanne A. I. Seidel,Patricia M. Dijkman,Wendy A. Lea,Geert van den Bogaart,Moran Jerabek-Willemsen,Ana Lazic,Jeremiah S. Joseph,Prakash Srinivasan,Philipp Baaske,Anton Simeonov,Ilia Katritch,Fernando A. Melo,John E. Ladbury,Gideon Schreiber,Anthony Watts,Dieter Braun,Stefan Duhr +16 more
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TLDR
Flexibility in assay design qualifies MST for analysis of biomolecular interactions in complex experimental settings, which is demonstrated by addressing typically challenging types of binding events from various fields of life science.About:
This article is published in Methods.The article was published on 2013-03-01 and is currently open access. It has received 508 citations till now. The article focuses on the topics: Microscale thermophoresis & Thermophoresis.read more
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Book ChapterDOI
Microscale Thermophoresis (MST ) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-Lipid Interactions.
TL;DR: This chapter demonstrates for the first time, the application of MST to the determination of DSP-lipid interactions and the accurate measurement of KD under physiologically relevant solution conditions.
Dissertation
Developing small molecule inhibitors of bromodomain-histone interactions
TL;DR: This work has provided useful structure-activity relationships for a subset of bromodomains, which will be useful in future ligand development programmes of this emerging therapeutic target class.
Journal ArticleDOI
Sulfoglycodendrimer Therapeutics for HIV-1 and SARS-CoV-2.
Lauren Wells,Cory Vierra,Janee’ Hardman,Yanxiao Han,Dustin Dimas,Lucia N. Gwarada‐Phillips,Rachel Blackeye,Daryl K. Eggers,Celia C. LaBranche,Petr Král,Katherine D. McReynolds +10 more
TL;DR: Hexavalent sulfoglycodendrimers (SGDs) are synthesized as mimics of host cell heparan sulfate proteoglycans (HSPGs) to inhibit the early stages in viral binding/entry of HIV-1 and SARS-CoV-2 as mentioned in this paper.
Journal ArticleDOI
Pentatricopeptide repeat protein CNS1 regulates maize mitochondrial complex III assembly and seed development.
Shuai Ma,Wenzhu Yang,Xiaoqing Liu,Suzhen Li,Ye Li,Jiameng Zhu,Chunyi Zhang,Xiaoduo Lu,Xiaojin Zhou,Rumei Chen +9 more
TL;DR: Results indicate that CNS1 is essential for reaching a suitable level of intact ccmFN transcripts through binding to the 5'-UTR of the RNAs and maintaining 5'-integrity, which is crucial for sustaining mitochondrial complex III function to ensure mitochondrial biogenesis and seed development in maize.
Journal ArticleDOI
Do interactions between protein and phospholipids influence the release behavior from lipid-based exenatide depot systems?
TL;DR: Erosion of the VPG matrix and membrane permeability of the drug are the major parameters influencing the release of exenatide from VPGs of POPC-POPG, whereas drug binding to the membranes had a minor effect only.
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