Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions
Susanne A. I. Seidel,Patricia M. Dijkman,Wendy A. Lea,Geert van den Bogaart,Moran Jerabek-Willemsen,Ana Lazic,Jeremiah S. Joseph,Prakash Srinivasan,Philipp Baaske,Anton Simeonov,Ilia Katritch,Fernando A. Melo,John E. Ladbury,Gideon Schreiber,Anthony Watts,Dieter Braun,Stefan Duhr +16 more
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TLDR
Flexibility in assay design qualifies MST for analysis of biomolecular interactions in complex experimental settings, which is demonstrated by addressing typically challenging types of binding events from various fields of life science.About:
This article is published in Methods.The article was published on 2013-03-01 and is currently open access. It has received 508 citations till now. The article focuses on the topics: Microscale thermophoresis & Thermophoresis.read more
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Biophysical characterization of laforin–carbohydrate interaction
David M. Dias,David M. Dias,Joana Furtado,Emeric Wasielewski,Rui Cruz,Bernard Costello,Lindsay Cole,Tiago Q. Faria,Philipp Baaske,Rui M. M. Brito,Alessio Ciulli,Isaura Simões,Sandra Macedo-Ribeiro,Carlos Faro,Carlos F. G. C. Geraldes,Pedro Castanheira +15 more
TL;DR: The work in the present study complements the structural characterization of la forin and sheds light on the molecular mechanism of laforin-glycan interaction, which is a pivotal requisite to understand the physiological and pathological roles of Laforin.
Journal ArticleDOI
Functional characterisation of G protein-coupled receptors.
TL;DR: This review focuses on G protein-coupled receptors (GPCRs), examining techniques that can be applied to both receptors in membranes and after solubilisation, and a variety of experimental techniques available to incorporate suitable donors and acceptors within the receptor.
Journal ArticleDOI
19F NMR as a Tool for Monitoring Individual Differentially Labeled Proteins in Complex Mixtures
TL;DR: This study proposes differential labeling of proteins by covalent attachment of 19F groups with distinct chemical shifts, giving each protein a unique spectral signature which can be monitored by 19F NMR without signal overlap, even in complex mixtures, and without any interfering signals from the buffer or other unlabeled components.
Journal ArticleDOI
Studying epigenetic interactions using MicroScale Thermophoresis (MST)
Thomas Schubert,Gernot Längst +1 more
TL;DR: The MicroScale Thermophoresis (MST) is a rapid and precise method to characterize epigenetic interactions in solution at microliter scale, requiring low concentrations of the potential interactors.
Book ChapterDOI
Biophysical Analysis of Sec-Mediated Protein Translocation in Nanodiscs
TL;DR: Cryo-electron microscopy of nanodisc-reconstituted SecYEG and YidC in complex with ribosomes visualized intermediates on membrane protein insertion and demonstrated structural dynamics of insertases, and suggestednanodiscs as a powerful platform for further studies, including high-resolution structural analysis.
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