Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions
Susanne A. I. Seidel,Patricia M. Dijkman,Wendy A. Lea,Geert van den Bogaart,Moran Jerabek-Willemsen,Ana Lazic,Jeremiah S. Joseph,Prakash Srinivasan,Philipp Baaske,Anton Simeonov,Ilia Katritch,Fernando A. Melo,John E. Ladbury,Gideon Schreiber,Anthony Watts,Dieter Braun,Stefan Duhr +16 more
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TLDR
Flexibility in assay design qualifies MST for analysis of biomolecular interactions in complex experimental settings, which is demonstrated by addressing typically challenging types of binding events from various fields of life science.About:
This article is published in Methods.The article was published on 2013-03-01 and is currently open access. It has received 508 citations till now. The article focuses on the topics: Microscale thermophoresis & Thermophoresis.read more
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JMJD5 couples with CDK9 to release the paused RNA polymerase II
Haolin Liu,Haolin Liu,Srinivas Ramachandran,Nova Fong,Tzu L. Phang,Schuyler Lee,Schuyler Lee,Pirooz V. Parsa,Xinjian Liu,Laura Harmacek,Thomas Danhorn,Tengyao Song,Sangphil Oh,Qianqian Zhang,Zhongzhou Chen,Qian Zhang,Ting-Hui Tu,Carrie Happoldt,Brian O’Conner,Ralf Janknecht,Chuan-Yuan Li,Philippa Marrack,Philippa Marrack,John W. Kappler,John W. Kappler,Sonia M. Leach,Gongyi Zhang,Gongyi Zhang +27 more
TL;DR: It is found that knockout of JMJD5 in mice leads to accumulation of nucleosomes at +1 and loss of expression of a large number of genes and phosphorylation, by CDK9, of Ser2 within two neighboring heptad repeats in the carboxyl-terminal domain of Pol II allows Pol II to bindJMJD5 via engagement of the N-terminAL domain of JM JD5.
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Notch Activation by Shootin1 Opposing Activities on 2 Ubiquitin Ligases.
Tamar Sapir,Talia Levy,Noga Kozer,Irina Shin,Vanessa Zamor,Rebecca Haffner-Krausz,Jane McGlade,Orly Reiner +7 more
TL;DR: It is identified that Shootin1 exhibits the surprising propensity of activating the Notch pathway either by interacting with LNX1/2 and promoting poly-ubiquitination of Numb or by complexing with Itch and impairing poly-UBiquitinations of NICD.
Journal ArticleDOI
Biophysical Characterisation and Quantification of Nucleic Acid-Protein Interactions: EMSA, MST and SPR.
Janine K. Flores,Ruvini Kariawasam,Adrian X. Gimenez,Stephanie Helder,Liza Cubeddu,Roland Gamsjaeger,Sandro F. Ataide +6 more
TL;DR: Three techniques that are commonly used to quantify and characterize DNA-protein and RNA-protein interactions are presented and their main advantages and limitations are discussed.
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Non-full-length Water-Soluble CXCR4QTY and CCR5QTY Chemokine Receptors: Implication for Overlooked Truncated but Functional Membrane Receptors.
Rui Qing,Fei Tao,Pranam Chatterjee,Gaojie Yang,Qiuyi Han,Haeyoon Chung,Jun Ni,Bernhard P. Suter,Jan Kubicek,Barbara Maertens,Thomas Schubert,Camron Blackburn,Shuguang Zhang +12 more
TL;DR: Insight is provided into essential structural components for CCR5 and CXCR4 functionality, while raising the possibility that non-full-length receptors may be resulted from alternative splicing and that pseudo-genes in genomes may be present and functional in living organisms.
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Tampering with Cell Division by Using Small‐Molecule Inhibitors of CDK–CKS Protein Interactions
Amel Hamdi,Aurélien Lesnard,Peggy Suzanne,Thomas Robert,Maria A. Miteva,Morgan Pellerano,Bruno Didier,Elizabeth Ficko-Blean,Annelise Lobstein,Marcel Hibert,Sylvain Rault,May C. Morris,Pierre Colas +12 more
TL;DR: Two small‐molecule inhibitors ofCDK–CKS interactions are discovered that should help decipher the complex contributions of CDK-CKS complexes in the regulation of cell division and might present an interesting therapeutic potential.
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