Miniaturized devices for isothermal DNA amplification addressing DNA diagnostics
Georgia D. Kaprou,George Papadakis,Dimitrios P. Papageorgiou,George Kokkoris,V. Papadopoulos,I. Kefala,Electra Gizeli,Angeliki Tserepi +7 more
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TLDR
This work presents a low-cost and fast DNA amplification device for isothermal helicase dependent amplification implemented in the detection of mutations related to breast cancer as well as the Detection of Salmonella pathogens.Abstract:
Microfluidics is an emerging technology enabling the development of lab-on-a-chip systems for clinical diagnostics, drug discovery and screening, food safety and environmental analysis. Currently, available nucleic acid diagnostic tests take advantage of polymerase chain reaction that allows exponential amplification of portions of nucleic acid sequences that can be used as indicators for the identification of various diseases. At the same time, isothermal methods for DNA amplification are being developed and are preferred for their simplified protocols and the elimination of thermocycling. Here, we present a low-cost and fast DNA amplification device for isothermal helicase dependent amplification implemented in the detection of mutations related to breast cancer as well as the detection of Salmonella pathogens. The device is fabricated by mass production amenable technologies on printed circuit board substrates, where copper facilitates the incorporation of on-chip microheaters, defining the thermal zone necessary for isothermal amplification methods.read more
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Microfluidic devices for forensic DNA analysis: A review
Brigitte Bruijns,Brigitte Bruijns,Arian C. van Asten,Arian C. van Asten,Roald M. Tiggelaar,Han Gardeniers +5 more
TL;DR: An extensive overview of microfluidic devices for cell lysis, DNA extraction and purification, DNA amplification and detection and analysis techniques for DNA is provided, including polymerase chain reaction (PCR) on-chip, digital PCR (dPCR), isothermal amplification on- chip, chip materials, integrated devices and commercially available techniques.
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Combination of Microfluidic Loop-Mediated Isothermal Amplification with Gold Nanoparticles for Rapid Detection of Salmonella spp. in Food Samples.
Alejandro Garrido-Maestu,Sarah Azinheiro,Joana Carvalho,Sara Abalde-Cela,Enrique Carbó-Argibay,Lorena Diéguez,Marek Piotrowski,Yury V. Kolen'ko,Marta Prado +8 more
TL;DR: This system is intended for low-cost, in situ, detection of different pathogens, as the proposed methodology can be extrapolated to different microorganisms.
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Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples
George Papadakis,Pavla Murasova,Audrey Hamiot,Katerina Tsougeni,Georgia D. Kaprou,Michael J. Eck,David Rabus,Zuzana Bílková,Bruno Dupuy,Gerhard Jobst,Angeliki Tserepi,Evangelos Gogolides,Electra Gizeli +12 more
TL;DR: The work presents the first reported Lab-on-Chip platform that comprises an acoustic device as the sensing element, exhibiting impressive analytical features, namely, an acoustic limit of detection of 2 cells/μl or 3 aM of the DNA target and ability to detect in a label-free manner dsDNA amplicons in impure samples.
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Rapid Methods for Antimicrobial Resistance Diagnostics.
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Lab-on-Chip platform and protocol for rapid foodborne pathogen detection comprising on-chip cell capture, lysis, DNA amplification and surface-acoustic-wave detection
Katerina Tsougeni,Georgia D. Kaprou,C.M. Loukas,George Papadakis,Audrey Hamiot,Michael J. Eck,David Rabus,George Kokkoris,Stavros Chatzandroulis,V. Papadopoulos,Bruno Dupuy,Gerhard Jobst,Electra Gizeli,Angeliki Tserepi,Evangelos Gogolides +14 more
TL;DR: Lab on a chip platform and protocol for rapid pathogen analysis in food samples based on an oxygen plasma nanotextured polymeric chip combining in one microfluidic chamber bacteria immunoaffinity capturing on the chip surface, chemical lysis and DNA isothermal amplification, followed by label-free detection with a Surface Acoustic Wave (SAW) biosensor.
References
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