Journal ArticleDOI
Mitochondrial genome rearrangement leads to extension and relocation of the cytochrome c oxidase subunit I gene in sorghum
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TLDR
The novel 9E COXI, apparently arising from at least two rearrangements, affects transcription and gene product.About:
This article is published in Cell.The article was published on 1986-11-21. It has received 116 citations till now. The article focuses on the topics: Cytochrome c oxidase subunit I & Gene rearrangement.read more
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Plant mitochondrial DNA evolved rapidly in structure, but slowly in sequence
TL;DR: The tempo and mode of mitochondrial DNA evolution in six species of crucifers from two genera, Brassica and Raphanus, are examined, finding the rate of rearrangements is extraordinarily faster in plant mtDNA than in cpDNA and animal mtDNA.
Journal ArticleDOI
Molecular biology of plant mitochondria
C. S. Levings,G.G. Brown +1 more
TL;DR: The mitochondrial genome of a liverwort, Marchantia polymorpha, and Aberrant growth phenotypes associated with mitochondrial genome rearrangements in higher plants are studied.
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Sequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica cybrids
Sandrine Bonhomme,Françoise Budar,Dominique Lancelin,Ian Small,Marie-Christine Defrance,Georges Pelletier +5 more
TL;DR: A tRNAfMet sequence, a putative 138 amino acid open reading frame (orf138), and a 158 amino acid ORF (orf158) previously observed in mitochondria) genomes from several other plant species are revealed.
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Suppression of cytoplasmic male sterility by nuclear genes alters expression of a novel mitochondrial gene region.
Mahipal Singh,Gregory G. Brown +1 more
TL;DR: Structural analysis of the atp6 gene regions of pol and nap mitochondrial DNAs showed that rearrangements in the pol mitochondrial genome occurring upstream of at p6 have generated a chimeric 224-codon open reading frame, designated orf224, that is cotranscribed with atp 6.
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Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene
TL;DR: The organization and expression of the mitochondrial (mt) genome of fertile, male-sterile and restored lines of Helianthus annuus and of H. petiolaris were compared to identify alterations which might lead to cytoplasmic male sterility (CMS).
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
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A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.