Journal ArticleDOI
Modification of GP63 genes from diverse species of Leishmania for expression of recombinant protein at high levels in Escherichia coli.
TLDR
Mouse monoclonal antibodies raised against purified Leishmania major rGP63 had equivalent immunoblotting characteristics for native GP63 and recombinant GP63 with respect to linear determinants on GP63 expressed in diverse species ofLeishmania.About:
This article is published in Molecular and Biochemical Parasitology.The article was published on 1991-02-01. It has received 42 citations till now. The article focuses on the topics: Leishmania braziliensis & Molecular cloning.read more
Citations
More filters
Journal ArticleDOI
Effective immunization against cutaneous leishmaniasis with recombinant bacille Calmette-Guérin expressing the Leishmania surface proteinase gp63
TL;DR: The results support the view that recombinant BCG expressing gp63 may prove a useful vaccine for inducing protective cell-mediated immune responses to Leishmania species causing American cutaneous leishmaniasis.
Journal ArticleDOI
Leishmania GP63 alters host signaling through cleavage-activated protein tyrosine phosphatases.
Maria Adelaida Gomez,Irazú Contreras,Irazú Contreras,Maxime Hallé,Michel L. Tremblay,Robert McMaster,Martin Olivier,Martin Olivier +7 more
TL;DR: The data present a mechanism of cleavage-dependent activation of macrophage PTPs by an obligate intracellular pathogen and show that internalization of GP63, a key Leishmania virulence factor, into host macrophages is a strategy the parasite uses to interact and survive within its host.
Journal ArticleDOI
Targeted gene deletion of Leishmania major genes encoding developmental stage‐specific leishmanolysin (GP63)
TL;DR: The highly expressed promastigote forms of GP63, encoded by gp63 genes 1–6, do not appear to be required for nutrient utilization in the bloodmeal during the early stages of development in the sandfly or for midgut attachment and further development.
Journal ArticleDOI
Expression of lipophosphoglycan, high-molecular weight phosphoglycan and glycoprotein 63 in promastigotes and amastigotes of Leishmania mexicana.
TL;DR: Immunoblotting experiments demonstrate that amastigotes synthesize hydrophilic high-molecular weight compounds which stain blue with Stains-all and cross-react with the monoclonal and polyvalent antibodies suggesting the presence of similar phosphoglycan structures as in LPG.
Journal ArticleDOI
Genetic immunization with glycoprotein 63 cDNA results in a helper T cell type 1 immune response and protection in a murine model of leishmaniasis.
Patricia S. Walker,Tanya Scharton-Kersten,Edgar D. Rowton,Ulrich R. Hengge,Anne Bouloc,Mark C. Udey,Jonathan C. Vogel +6 more
TL;DR: It is concluded that gp63-pcDNA-3 genetic vaccination results in a CD4-dependent Th1 immune response that correlates with protection from disease, and suggest that skin-derived dendritic cells are involved in priming this response.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Journal ArticleDOI
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Randall Keichi Saiki,Stephen J. Scharf,Fred A. Faloona,Kary B. Mullis,Glenn Thomas Horn,Henry A. Erlich,Norman Arnheim +6 more
TL;DR: Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia, using primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase of target DNA copies.
Related Papers (5)
The involvement of the major surface glycoprotein (gp63) of Leishmania promastigotes in attachment to macrophages.
D G Russell,H Wilhelm +1 more
Effective immunization against cutaneous leishmaniasis with defined membrane antigens reconstituted into liposomes.
D G Russell,J Alexander +1 more