Showing papers in "Molecular and Biochemical Parasitology in 1993"

TL;DR: This poster presents a probabilistic procedure to quantify the numbers of Gram-positive and Gram-negative parasites found in the blood of Malaria patients infected with E.coli.

TL;DR: Genus- and species-specific sequences are present within the small subunit ribosomal RNA genes of the four human malaria parasites that have proven to be more sensitive and accurate than by routine diagnostic microscopy.

TL;DR: All published sequences of MSP-1 are aligned, identifying errors, resequencing a portion of one parasite clone, and identifying probable duplicate sequences of four pairs of parasite clones to serve as a data base for planning vaccine trials.

TL;DR: This method offers an objective, simple, highly sensitive and rapid approach for the discrimination of Echinococcus isolates and for study of other parasite complexes.

TL;DR: It was found that, unlike bacterial genomic DNA, chromosomal DNA of trypanosomatids was soluble in the phenol/chloroform/Triton/LiCl mixture, which prompted the use of the bacterial lysis method as a routine protocol for extraction of DNA from trypanOSomatid protozoa.

TL;DR: Analysis of pfmdr 1 in recent isolates from Thailand revealed an apparent association between increased gene copy number and increased level of expression of pFMdr 1 and decreased susceptibility to mefloquine and halofantrine.

TL;DR: The results suggest that reactive oxygen species are produced in the parasite's food vacuole during the digestion of host cell cytosol, and are able to egress from the parasite to the host cell compartment.

TL;DR: A phylogenetic analysis of the small subunit ribosomal RNA (16S-like rRNA) coding region from Hexamita inflata demonstrates that parasitism alone cannot explain early diverging eukaryotic lineages.

TL;DR: This work shows that when metacyclic trypomastigotes are incubated with [3H]sialyllactose, most of the sialic acid is transferred to these 35/50-kDa molecules in a reaction catalyzed by a parasite transsialidase, indicating that these glycoproteins are anchored to the membrane by glycosylphosphatidylinositol.

TL;DR: In vitro cell attachment assays show that the cathepsin L-like proteinase purified from the E/S products of adult F. hepatica can prevent the antibody-mediated attachment of eosinophil to NEJ, indicating that this proteinase has an important biological function in immune evasion.

TL;DR: The sexual stage-specific protein Pbs21 of the rodent malaria parasite Plasmodium berghei, expressed on the surface of zygotes and ookinetes, has been shown to induce an effective and long-lasting transmission blocking immunity.

TL;DR: Immunolocalisation studies revealed that the cathepsin L-like proteinase is concentrated within vesicles in the gut epithelial cells of liver fluke.

TL;DR: It is shown by direct expression of the individual EGF-like domains in Escherichia coli, that the first domain is the target of growth-inhibitory antibodies and a single amino acid difference influences the binding of some antibodies to this domain.

TL;DR: It is proposed that the enzyme functions in intracellular protein degradation in some stages of T. cruzi, but also in remodeling of the parasite during transformation between stages, suggesting that inhibition of the T.cruzi cysteine protease is a potential lead for new chemotherapy of Chagas' disease.

TL;DR: It was shown that the target sequence is specific for this parasite and no cross-reactivity was observed with different species of pathogenic Leishmania, two strains of Trypanosoma rangeli or human RNA.

TL;DR: Analysis of band sharing using bands present in all strains of a given zymodeme showed ZB and ZC to be closely related and Z1 and Z2 to form distinct groups.

TL;DR: The gene encoding the gametocyte/gamete-specific membrane protein Pfs48/45 of Plasmodium falciparum has been cloned and is recognized by transmission-blocking anti-45/48-kDa monoclonal antibodies.

TL;DR: A beta-tubulin isotype 1 gene from a benzimidazole (BZ)-resistant population of the nematode parasite, Haemonchus contortus, was cloned and sequenced and 3 mutations that could be involved in BZ resistance were identified.

TL;DR: Immunoblotting experiments demonstrate that amastigotes synthesize hydrophilic high-molecular weight compounds which stain blue with Stains-all and cross-react with the monoclonal and polyvalent antibodies suggesting the presence of similar phosphoglycan structures as in LPG.

TL;DR: Multivariate analysis suggests that Sm28GST has an overall catalytic homology with mammalian Mu class GSTs, whilst the enzymatic properties of Sj26GST appear to constitute a hybridisation of Mu and Alpha class features.

TL;DR: The results suggest that the trans-sialidase functions as a counter-receptor for trypomastigote binding to alpha 2,3-sIALyl receptors on host cells as a prelude to T. cruzi invasion.

TL;DR: A axenic culture system which permits the continuous generation and cycling of Leishmania donovani from promastigotes to 'amastigote' in vitro and revealed that the 3 cDNAs represent unique leishmanial genes.

TL;DR: The presumptive mitochondrial DNA, the 6-kb element, of Plasmodium falciparum is sequenced, permitting an analysis of the predicted structure of parasite electron transport proteins, suggesting that the same features are responsible for increased affinity of the parasite cyt b for antimalarial compounds of class 8-aminoquinolines and hydroxynaphthoquinones, explaining the therapeutic value of these drugs.

TL;DR: The cloning of the gene encoding the P21 antigen, for which the name GRA 5 is proposed, is reported, with deduced amino acid sequence of an open reading frame encoding 133 amino acids perfectly matched that of 5 peptides microsequenced from the native antigen.

TL;DR: The results indicate that the random amplification of polymorphic DNA (RAPD) may be an extremely useful approach to the identification of schistosome strains and species.

TL;DR: Analysis of phylogenetic affinities of the genus Perkinsus indicates that Perkinsus is phylogenetically closer to dinoflagellates and to coccidean and piroplasm apicomplexans than to fungi or flageLLates.

TL;DR: The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma, indicating clearly that RAPD markers constitute a powerful tool for the analysis of genetic variability.

TL;DR: The monosaccharide composition of an affinity-purified family of antigenically-related Trichinella spiralis larval glycoproteins was determined by gas chromatography/mass spectrometry and a 3,6-dideoxyhexose was identified, the same as found in Ascaris eggs.

TL;DR: A series of Trypanosoma brucei transfection vectors was constructed in which transcription of the luciferase gene was driven by the procyclic acidic repetitive protein (procyclin) promoter, and the nature of the 3'-untranslated region was crucial to the level of expression in bloodstream forms.