Book ChapterDOI
Modulating and targeting meiotic double-strand breaks in Saccharomyces cerevisiae.
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TLDR
The methods used in budding yeast to modulate locally and globally the native DSB frequencies are reviewed, including a powerful method to target Spo11-dependent meiotic DSB in novel chromosomal regions.Abstract:
Meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the evolutionary conserved Spo11 protein. Along the S. cerevisiae chromosomes, the DSB sites are not evenly distributed and the cleavage frequencies vary 10-100-fold from site to site. Herein are reviewed the methods used in budding yeast to modulate locally and globally the native DSB frequencies, including a powerful method to target Spo11-dependent meiotic DSB in novel chromosomal regions. These methods serve to investigate the control and the mechanism of recombination initiation and modify the natural distribution of meiotic recombination.read more
Citations
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Journal ArticleDOI
The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair.
Maria Jasin,James E. Haber +1 more
TL;DR: DSB repair mechanisms and site-specific cleavage systems that have provided insight into these mechanisms and led to the current gene editing revolution are reviewed.
Journal ArticleDOI
Programming sites of meiotic crossovers using Spo11 fusion proteins.
Roberta Sarno,Roberta Sarno,Yoan Vicq,Yoan Vicq,Norio Uematsu,Norio Uematsu,Marine Luka,Marine Luka,Clement Lapierre,Clement Lapierre,Dana Carroll,Giacomo Bastianelli,Alexandre Serero,Alexandre Serero,Alain Nicolas,Alain Nicolas +15 more
TL;DR: New generic experimental strategies to increase the genetic diversity of gametes are described, which should prove useful in plant breeding and other applications.
Journal ArticleDOI
DNA repair systems
TL;DR: The basic molecular mechanisms underlying the rela vely rare monogenic diseases of DNA repair and management of genome integrity as well as the common mul factorial diseases and condi ons with late onset that are associated with increased levels of oxida ve stress are analyzed.
Využití imunofluorescence a fluorescenční in situ hybridizace pro studium meiotických a mitotických chromozomů u rodiny Cetartiodactyla
TL;DR: Cetartiodactyla je v soucasnosti jedna z největsich skupin savců na zemi, ktera zahrnuje 10 celedi suchozemských a 12 celedi vodnich savCů, while celedi Bovidae existuje znacna variabilita v diploidnim poctu chromozomů.
References
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Book ChapterDOI
Mechanism and control of meiotic recombination initiation.
TL;DR: This chapter reviews the properties of Spo11 and the other gene products required for meiotic DSB formation in a number of organisms and discusses ways in which recombination initiation is coordinated with other events occurring in the meiotic cell.
Journal ArticleDOI
Meiotic recombination hot spots and cold spots
TL;DR: The authors showed that the distribution of recombination events in eukaryotic genomes might reflect global features of chromosome structure, such as distribution of modified nucleosomes, which can hinder the ability of geneticists to identify genes by map-based techniques.
Journal ArticleDOI
Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains
Jeff Smith,Marina Bibikova,Frank G. Whitby,Attipalli Ramachandra Reddy,Attipalli Ramachandra Reddy,Srinivasan Chandrasegaran,Dana Carroll +6 more
TL;DR: The results define requirements for effective targets of chimeric nucleases and will guide the design of novel specificities for directed DNA cleavage in vitro and in vivo.
Journal ArticleDOI
Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae
Jennifer L. Gerton,Joseph L. DeRisi,Robert Shroff,Michael Lichten,Patrick O. Brown,Thomas D. Petes +5 more
TL;DR: This work used DNA microarrays to estimate variation in the level of nearby meiotic DSBs for all 6,200 yeast genes and found hotspots and coldspots were nonrandomly associated with regions of high G + C base composition and certain transcriptional profiles.
Journal ArticleDOI
Meiotic Recombination Hotspots
TL;DR: This article reviews what is known about meiotic recombination hotspots loci, or regions that display a greater than average frequency of meiotic exchange, in Saccharomyces cerevisiae.