Modulatory effects of lipoic acid and selenium against cadmium-induced biochemical alteration in testicular steroidogenesis
TLDR
The beneficial influences of lipoic acid and selenium were demonstrated in reducing harmful effects of Cd2+ in rats' testes and were effective in modulation of most of the measured biochemical parameters.Abstract:
Exposure to toxic metals including cadmium has become an increasingly recognized source of illness worldwide. Cadmium (Cd2+) is one of the environmental pollutants affecting various tissues and organs including testis. The protective effect of lipoic acid and selenium on Cd2+‐induced testicular damage was investigated. Accordingly, male Wistar rats were allocated into four groups (n = 8; each). Gp I: (control), whereas the other 3 groups received CdCl2 (2 mg/kg, i.p. for 28 days) alone or in combination with either (i) lipoic acid (35 mg/kg, p.o) or (ii) selenium (0.35 mg/kg, p.o) throughout the experiment. Serum testosterone, luteinizing hormone and follicle‐stimulating hormone levels significantly decreased in the Cd2+‐exposed rats. The activities of testicular key androgenic enzymes, 3β‐hydroxysteroid dehydrogenase and 17 β‐HSD significantly decreased in Cd2 exposed rats compared to the control counterparts. In addition, the activities of testicular marker enzymes were significantly altered in cadmium‐treated animals. Significant reductions in body and testicular weight as well as antioxidant status were also observed in Cd2+‐exposed rats. Moreover, some testicular metal levels were altered. Lipoic acid and selenium significantly increased serum testosterone level and restored testicular activity of 3β‐HSD and 17 β‐HSD and were effective in modulation of most of the measured biochemical parameters. The biochemical parameters were further confirmed with histopathological findings. In conclusion, the present study demonstrated the beneficial influences of lipoic acid and selenium in reducing harmful effects of Cd2+ in rats' testes. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:15–25 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20354read more
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References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power' : the FRAP assay
TL;DR: The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.
Journal ArticleDOI
Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase
TL;DR: Glutathione peroxidase activity is found to be associated with a relatively stable, nondialyzable, heat-labile, intracellular component which is separable from hemoglobin, by gel filtration and ammonium sulfate precipitation.
Journal ArticleDOI
A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
Stanley Reitman,Sam Frankel +1 more
Journal Article
Improved method for the determination of blood glutathione.
Ernest Beutler,O Duron,B M Kelly +2 more