Molecular analysis and epidemiological typing of Vancomycin-resistant Enterococcus outbreak strains
Anbjørg Rangberg,Astri Lervik Larsen,Oliver Kacelnik,Hanne Skarpodde Sæther,Marthe Bjørland,Jetmund Ringstad,Christine M. Jonassen +6 more
TLDR
Despite lower resolution compared to PFGE, MALDI-TOF may provide an efficient mean for real-time monitoring spread of infection, as it is shown to provide a strong correlation with PFGE.Abstract:
Outbreaks of multidrug resistant bacteria including vancomycin-resistant enterococci (VRE) in healthcare institutions are increasing in Norway, despite a low level of resistance compared to other European countries. In this study, we describe epidemiological relatedness of vancomycin-resistant Enterococcus faecium isolated during an outbreak at a Norwegian hospital in 2012–2013. During the outbreak, 9454 fecal samples were screened for VRE by culture and/or PCR. Isolates from 86 patients carrying the vanA resistance gene were characterized using pulsed-field gel electrophoresis (PFGE), MALDI-TOF mass spectrometry and single nucleotide polymorphism typing. PFGE revealed two main clusters, the first comprised 56 isolates related to an initial outbreak strain, and the second comprised 21 isolates originating from a later introduced strain, together causing two partly overlapping outbreaks. Nine isolates, including the index case were not related to the two outbreak clusters. In conclusion, the epidemiological analyses show that the outbreak was discovered by coincidence, and that infection control measures were successful. All typing methods identified the two outbreak clusters, and the experiment congruence between the MALDI-TOF and the PFGE clustering was 63.2%, with a strong correlation (r = 72.4%). Despite lower resolution compared to PFGE, MALDI-TOF may provide an efficient mean for real-time monitoring spread of infection.read more
Citations
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Pop-up paper-based and fully integrated microdevice for point-of-care testing of vancomycin-resistant Enterococcus
TL;DR: A highly sensitive detection with the limit of VRE detection of 102 CFU/mL in milk was achieved using this portable and integrated microdevice, which holds significant promise for point-of-care applications to accurately and rapidly diagnose and control VRE.
Journal ArticleDOI
Vancomycin-resistant Enterococcus faecium colonizing patients on hospital admission in Germany: prevalence and molecular epidemiology.
Kyriaki Xanthopoulou,Silke Peter,David Tobys,Michael Behnke,Ariane G Dinkelacker,Simone Eisenbeis,Jane Falgenhauer,Linda Falgenhauer,Moritz Fritzenwanker,Hannah Gölz,Georg Häcker,Paul G. Higgins,Can Imirzalioglu,Nadja Käding,Winfried V. Kern,Evelyn Kramme,Axel Kola,Alexander Mischnik,Siegbert Rieg,Anna M Rohde,Jan Rupp,Evelina Tacconelli,Maria J G T Vehreschild,Maria J G T Vehreschild,Sarah V Walker,Petra Gastmeier,Harald Seifert +26 more
TL;DR: These data provide insight into the epidemiology of VREfm at six German university hospitals and demonstrate the remarkable inter-regional clonal expansion of the ST117/vanB V REfm clone.
Journal ArticleDOI
Application of MALDI-TOF MS for the subtyping of Arcobacter butzleri strains and comparison with their MLST and PFGE types
Federica Giacometti,Silvia Piva,Katleen Vranckx,Katrien De Bruyne,Ilenia Drigo,Alex Lucchi,Gerardo Manfreda,Andrea Serraino +7 more
TL;DR: MALDI-TOF appears to be a relatively low cost answer to the urgent need for more rapid, less expensive typing tools suitable for source attribution studies, readily allowing multiple typing methods to be combined.
Journal ArticleDOI
Molecular determination of van genes among clinical isolates of enterococci at a hospital setting.
Siju K. George,M. R. Suseela,Saleh El Safi,Elmoeiz A Elnagi,Yaser A Al-Naam,Ahmed Adlan Mohammed Adam,Ashly Mary Jacob,Thekra N Al-Maqati,Harish Kumar Ks +8 more
TL;DR: This study detected a low prevalence of VRE among clinical isolates of enterococci and that the vanB gene predominates in such strains and indicated that linezolid and tigecycline are still effective against these multidrug-resistant pathogens.
Journal ArticleDOI
Spinning and Fully Integrated Microdevice for Rapid Screening of Vancomycin-Resistant Enterococcus.
Thi Ngoc Diep Trinh,Nae Yoon Lee +1 more
TL;DR: In this article, a spinning and fully integrated paper-based microdevice that can perform multiple functions, including DNA extraction, amplification, and colorimetric detection, for monitoring two major vancomycin-resistant Enterococci (VREs), which carry the vanA and vanB genes.
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