Molecular cloning of a cysteine proteinase inhibitor of rice (oryzacystatin). Homology with animal cystatins and transient expression in the ripening process of rice seeds.
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TLDR
A cDNA clone for a cysteine proteinase inhibitor of rice (oryzacystatin) was isolated from a lambda gt10 cDNA library of rice immature seeds by screening with synthesized oligonucleotide probes based on partial amino acid sequences of oryzacyStatin.About:
This article is published in Journal of Biological Chemistry.The article was published on 1987-12-15 and is currently open access. It has received 273 citations till now. The article focuses on the topics: cDNA library & Complementary DNA.read more
Citations
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Journal ArticleDOI
The cystatins: protein inhibitors of cysteine proteinases.
Vito Turk,Wolfram Bode +1 more
TL;DR: Recently determined crystal structures of chicken cystatin and human stefin B established a new mechanism of interaction between cysteine proteinases and their inhibitors which is fundamentally different from the standard mechanism for serine proteinase inhibitors and their inhibitor.
Patent
Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof
Thomas R. Adams,Sheryl A. Chambers,Richard J. Daines,William J. Gordon-Kamm,Albert P. Kausch,Peggy G. Lemaux,Catherine J. Mackey,Mary L. Mangano,James V. O'brien,Thomas B. Rice,T. Michael Spencer,William G. Start,Nancy Willetts +12 more
TL;DR: In this article, a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed, is described. But the system is not applicable to the field of agriculture.
PatentDOI
Fertile transgenic corn plants
TL;DR: Fertile transgenic Zea mays (corn) plants which stably express recombinant DNA which is heritable are provided wherein said DNA preferably comprises a recombinant gene which encodes a seed storage protein, so that the amino acid profile of the corn is improved as discussed by the authors.
Journal ArticleDOI
Plant protease inhibitors in control of phytophagous insects
TL;DR: The classes of protease inhibitors, their regulation and genes used to construct transgenic plants against phytophagous insects are described.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
Haim Aviv,Philip Leder +1 more
TL;DR: A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.
Journal ArticleDOI
A simple and very efficient method for generating cDNA libraries
Ueli Gubler,Beth J. Hoffman +1 more
TL;DR: Using the fully sequenced 1300 nucleotide-long bovine preproenkephalin mRNA, it is established by sequencing that the method yields faithful full-length transcripts.