Journal ArticleDOI
Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast.
Michael Wang,Randall R. Reed +1 more
TLDR
The Olf-1 protein, expressed exclusively in the olfactory receptor neurons and their precursors, contains a new helix–loop–helix motif and functions as an apparent homodimer.Abstract:
A novel genetic selection in yeast has been used to isolate a complementary DNA for the transcriptional activator, Olf-1, which binds to the regulatory sequences of several olfactory-specific genes. The Olf-1 protein, expressed exclusively in the olfactory receptor neurons and their precursors, contains a new helix–loop–helix motif and functions as an apparent homodimer. Olf-1 may be the first member of a family of related proteins that may direct cellular differentiation in a variety of neuronal tissues.read more
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A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
Peter Uetz,Loic Giot,Gerard Cagney,Traci A. Mansfield,Richard S. Judson,James R. Knight,Daniel Lockshon,Vaibhav A. Narayan,Maithreyan Srinivasan,Pascale Pochart,Alia Qureshi-Emili,Ying Li,Brian C. Godwin,Diana Conover,Theodore S. Kalbfleisch,Govindan Vijayadamodar,Meijia Yang,Mark Johnston,Stanley Fields,Jonathan M. Rothberg +19 more
TL;DR: Examination of large-scale yeast two-hybrid screens reveals interactions that place functionally unclassified proteins in a biological context, interactions between proteins involved in the same biological function, and interactions that link biological functions together into larger cellular processes.
Journal ArticleDOI
The molecular basis of dehydration tolerance in plants
J. Ingram,Dorothea Bartels +1 more
TL;DR: A large number of genes with a potential role in drought tolerance have been described, and major themes in the molecular response have been established.
Journal ArticleDOI
REST: A mammalian silencer protein that restricts sodium channel gene expression to neurons
Jayhong A. Chong,José Tapia-Ramírez,Sandra Kim,Juan José Toledo-Aral,Yingcong Zheng,Michael Boutros,Yelena M. Altshuller,Michael A. Frohman,Susan D. Kraner,Gail Mandel +9 more
TL;DR: It is proposed that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST.
Journal ArticleDOI
The two-hybrid system: an assay for protein-protein interactions
Stanley Fields,Rolf Sternglanz +1 more
TL;DR: The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions that has been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.
Journal ArticleDOI
A transcriptional partner for MAD proteins in TGF-beta signalling.
TL;DR: A model is proposed to explain how TGF-β superfamily signals might regulate the expression of specific genes in the early embryo.
References
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Journal ArticleDOI
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
TL;DR: A procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters, including tRNA and Ad 2 VA, is developed.
Journal ArticleDOI
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Robert S. Sikorski,Philip Hieter +1 more
TL;DR: A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
Journal Article
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Journal ArticleDOI
A novel genetic system to detect protein-protein interactions.
Stanley Fields,Ok-kyu Song +1 more
TL;DR: A novel genetic system to study protein-protein interactions between two proteins by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae, which may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.
Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.
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