Monitoring microevolution of OXA-48-producing Klebsiella pneumoniae ST147 in a hospital setting by SMRT sequencing.
Andreas E. Zautner,Boyke Bunk,Yvonne Pfeifer,Cathrin Spröer,Utz Reichard,Helmut Eiffert,Simone Scheithauer,Uwe Groß,Jörg Overmann,Wolfgang Bohne +9 more
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TLDR
Single molecule real-time sequencing allowed monitoring of the genetic and epigenetic microevolution of MDR OXA-48-producing K. pneumoniae and revealed in addition to SNPs, complex rearrangements of genetic elements.Abstract:
Objectives Carbapenemase-producing Klebsiella pneumoniae pose an increasing risk for healthcare facilities worldwide. A continuous monitoring of ST distribution and its association with resistance and virulence genes is required for early detection of successful K. pneumoniae lineages. In this study, we used WGS to characterize MDR blaOXA-48-positive K. pneumoniae isolated from inpatients at the University Medical Center Gottingen, Germany, between March 2013 and August 2014. Methods Closed genomes for 16 isolates of carbapenemase-producing K. pneumoniae were generated by single molecule real-time technology using the PacBio RSII platform. Results Eight of the 16 isolates showed identical XbaI macrorestriction patterns and shared the same MLST, ST147. The eight ST147 isolates differed by only 1-25 SNPs of their core genome, indicating a clonal origin. Most of the eight ST147 isolates carried four plasmids with sizes of 246.8, 96.1, 63.6 and 61.0 kb and a novel linear plasmid prophage, named pKO2, of 54.6 kb. The blaOXA-48 gene was located on a 63.6 kb IncL plasmid and is part of composite transposon Tn1999.2. The ST147 isolates expressed the yersinabactin system as a major virulence factor. The comparative whole-genome analysis revealed several rearrangements of mobile genetic elements and losses of chromosomal and plasmidic regions in the ST147 isolates. Conclusions Single molecule real-time sequencing allowed monitoring of the genetic and epigenetic microevolution of MDR OXA-48-producing K. pneumoniae and revealed in addition to SNPs, complex rearrangements of genetic elements.read more
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The Global Ascendency of OXA-48-Type Carbapenemases.
Johann D. D. Pitout,Gisele Peirano,Marleen M. Kock,Marleen M. Kock,Kathy-Anne Strydom,Kathy-Anne Strydom,Yasufumi Matsumura +6 more
TL;DR: Overall, clonal dissemination plays a minor role in the spread of OXA-48-like carbapenemases, but certain high-risk clones (e.g., Klebsiella pneumoniae sequence type 147 [ST147], ST307, ST15, and ST14 and Escherichia coli ST38 and ST410) have been associated with the global dispersion of Oxton-48, OxA-181, OXa-232, and O
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Emerging Antimicrobial-Resistant High-Risk Klebsiella pneumoniae Clones ST307 and ST147
TL;DR: ST307 and ST147 have the ability to become major threats to public health due to their worldwide distribution, ability to cause serious infections, and association with AMR, including panresistance.
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Phage Therapy: What Have We Learned?
Andrzej Górski,Ryszard Międzybrodzki,Ryszard Międzybrodzki,Małgorzata Łobocka,Aleksandra Głowacka-Rutkowska,Agnieszka Bednarek,Jan Borysowski,Ewa Jończyk-Matysiak,Marzanna Łusiak-Szelachowska,Beata Weber-Dąbrowska,Natalia Bagińska,Sławomir Letkiewicz,Krystyna Dąbrowska,Jacques Scheres +13 more
TL;DR: The shift in position of the authorities, academia, media, non-governmental organizations, regulatory agencies, patients, and doctors which could enable further advances in the research and application of phage therapy are discussed.
Journal ArticleDOI
Genome-based analysis of Carbapenemase-producing Klebsiella pneumoniae isolates from German hospital patients, 2008-2014
Laura Becker,Martin Kaase,Yvonne Pfeifer,Stephan Fuchs,Annicka Reuss,Anja von Laer,Muna Abu Sin,Miriam Korte-Berwanger,Sören Gatermann,Guido Werner +9 more
TL;DR: The authors' study results suggest prevalence of “classical” K. pneumoniae strain types associated with widely distributed carbapenemase genes such as ST258/KPC-2 or ST512/ KPC-3 also in Germany is highly worrisome and requires intense molecular surveillance.
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Tracking microevolution events among ST11 carbapenemase-producing hypervirulent Klebsiella pneumoniae outbreak strains.
TL;DR: Emergence of colistin-resistant and carbapenemase-producing hypervirulent K. pneumoniae strains further emphasizes the urgency for the establishment of a coordinated global program to eradicate hypervirulence and/or pan-drug-resistant strains of K. tuberculosis from clinical settings and the community.
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