Multimodality Molecular Imaging of Glioblastoma Growth Inhibition with Vasculature-Targeting Fusion Toxin VEGF121/rGel

TLDR: The results of this study suggest that future clinical multimodality imaging and therapy with VEGF(121)/rGel may provide an effective means to prospectively identify patients who will benefit from VEG fusions therapy and then stratify, personalize, and monitor treatment to obtain optimal survival outcomes.

Abstract: Vascular endothelial growth factor A (VEGF-A) and its receptors, Flt-1/FLT-1 (VEGFR-1) and Flk-1/KDR (VEGFR-2), are key regulators of tumor angiogenesis and tumor growth. The purpose of this study was to determine the antiangiogenic and antitumor efficacies of a vasculature-targeting fusion toxin (VEGF121/rGel) composed of the VEGF-A isoform VEGF121 linked with a G4S tether to recombinant plant toxin gelonin (rGel) in an orthotopic glioblastoma mouse model by use of noninvasive in vivo bioluminescence imaging (BLI), MRI, and PET. Methods: Tumor-bearing mice were randomized into 2 groups and balanced according to BLI and MRI signals. PET with 64Cu-1,4,7,10-tetraazacyclododedane-N,N′,N″,N‴-tetraacetic acid (DOTA)-VEGF121/rGel was performed before VEGF121/rGel treatment. 18F-Fluorothymidine (18F-FLT) scans were obtained before and after treatment to evaluate VEGF121/rGel therapeutic efficacy. In vivo results were confirmed with ex vivo histologic and immunohistochemical analyses. Results: Logarithmic transformation of peak BLI tumor signal intensity revealed a strong correlation with MRI tumor volume (r = 0.89, n = 14). PET with 64Cu-DOTA-VEGF121/rGel before treatment revealed a tumor accumulation (mean ± SD) of 11.8 ± 2.3 percentage injected dose per gram at 18 h after injection, and the receptor specificity of the tumor accumulation was confirmed by successful blocking of the uptake in the presence of an excess amount of VEGF121. PET with 18F-FLT revealed significant a decrease in tumor proliferation in VEGF121/rGel-treated mice compared with control mice. Histologic analysis revealed specific tumor neovasculature damage after treatment with 4 doses of VEGF121/rGel; this damage was accompanied by a significant decrease in peak BLI tumor signal intensity. Conclusion: The results of this study suggest that future clinical multimodality imaging and therapy with VEGF121/rGel may provide an effective means to prospectively identify patients who will benefit from VEGF121/rGel therapy and then stratify, personalize, and monitor treatment to obtain optimal survival outcomes.