Journal ArticleDOI
Multiparent intercross populations in analysis of quantitative traits
TLDR
Limitations with multiparent intercross populations are that they require longer time and more resource to be generated and they are likely to show extensive segregation for developmental traits, limiting their use in the analysis of complex traits.Abstract:
Most traits of interest to medical, agricultural and animal scientists show continuous variation and complex mode of inheritance. DNA-based markers are being deployed to analyse such complex traits, that are known as quantitative trait loci (QTL). In conventional QTL analysis, F2, backcross populations, recombinant inbred lines, backcross inbred lines and double haploids from biparental crosses are commonly used. Introgression lines and near isogenic lines are also being used for QTL analysis. However, such populations have major limitations like predominantly relying on the recombination events taking place in the F1 generation and mapping of only the allelic pairs present in the two parents. The second generation mapping resources like association mapping, nested association mapping and multiparent intercross populations potentially address the major limitations of available mapping resources. The potential of multiparent intercross populations in gene mapping has been discussed here. In such populations both linkage and association analysis can be conductted without encountering the limitations of structured populations. In such populations, larger genetic variation in the germplasm is accessed and various allelic and cytoplasmic interactions are assessed. For all practical purposes, across crop species, use of eight founders and a fixed population of 1000 individuals are most appropriate. Limitations with multiparent intercross populations are that they require longer time and more resource to be generated and they are likely to show extensive segregation for developmental traits, limiting their use in the analysis of complex traits. However, multiparent intercross population resources are likely to bring a paradigm shift towards QTL analysis in plant species.read more
Citations
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Next-generation phenotyping: requirements and strategies for enhancing our understanding of genotype–phenotype relationships and its relevance to crop improvement
Joshua N. Cobb,Joshua N. Cobb,Genevieve DeClerck,Anthony J. Greenberg,Randy T. Clark,Randy T. Clark,Susan R. McCouch +6 more
TL;DR: Within this framework, the objective of modern phenotyping is to increase the accuracy, precision and throughput of phenotypic estimation at all levels of biological organization while reducing costs and minimizing labor through automation, remote sensing, improved data integration and experimental design.
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The journal of genetics
TL;DR: This is the oldest English language journal in genetics and retains its traditional interest in evolutionary research that is of relevance to geneticists, even if not explicitly genetic in nature.
Journal ArticleDOI
Genome-Wide Association Mapping for Seedling and Adult Plant Resistance to Stripe Rust in Synthetic Hexaploid Wheat
TL;DR: The results confirm that resynthesized wheat involving its progenitor species is a rich source of new stripe (yellow) rust resistance that may be useful in choosing SHWs and incorporating diverse yellow rust (YR) resistance loci into locally adapted wheat cultivars.
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Potential of a tomato MAGIC population to decipher the genetic control of quantitative traits and detect causal variants in the resequencing era
Laura Pascual,Nelly Desplat,Bevan Emma Huang,Aurore Desgroux,Laure Bruguier,Jean-Paul Bouchet,Quang Hien Le,Betty Chauchard,Philippe Verschave,Mathilde Causse +9 more
TL;DR: The first tomato MAGIC population is presented and its potential for the valorization of intraspecific variation, QTL mapping and causal polymorphism identification is highlighted, representing a new permanent resource for the tomato genetics community.
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The International Oryza Map Alignment Project: development of a genus-wide comparative genomics platform to help solve the 9 billion-people question
TL;DR: The wild relatives of rice contain a virtually untapped reservoir of traits that can be used help drive the 21st century green revolution and the urgent need for sustainable in situ conservation of the Oryza genus is discussed.
References
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Journal ArticleDOI
R/qtl: QTL mapping in experimental crosses
TL;DR: R/qtl is an extensible, interactive environment for mapping quantitative trait loci (QTLs) in experimental populations derived from inbred lines and includes functions for estimating genetic maps, identifying genotyping errors, and performing single-QTL and two-dimensional, two- QTL genome scans by multiple methods.
Journal ArticleDOI
Genome-wide association studies for common diseases and complex traits
TL;DR: Genome-wide association studies will soon become possible, and could open new frontiers in the understanding and treatment of disease, however, the execution and analysis of such studies will require great care.
Journal ArticleDOI
Genome-wide genetic marker discovery and genotyping using next-generation sequencing.
TL;DR: Best practices for several NGS methods for genome-wide genetic marker development and genotyping that use restriction enzyme digestion of target genomes to reduce the complexity of the target.
Book
Genetical implications of the structure of deoxyribonucleic acid
James D. Watson,Francis Crick +1 more
TL;DR: J. D. Watson, F. H. C. Crick: Genetical Implications of the Structure of Deoxyribonucleic Acid, 2nd Ed.
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