Journal ArticleDOI
Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp.
Laura Pierdomenico,Laura Bonsi,Mario Calvitti,Damiano Rondelli,Mario Arpinati,Gabriella Chirumbolo,Ennio Becchetti,Cosetta Marchionni,Francesco Alviano,Valentina Fossati,N. Staffolani,Michele Franchina,Alberto Grossi,Gian Paolo Bagnara +13 more
TLDR
Dental pulp is an easily accessible and efficient source of MSCs, with different kinetics and differentiation potentialities from MSCS as isolated from the bone marrow, and the rapid proliferative capacity together with the immunoregulatory characteristics of DP-MSCs may prompt future studies aimed at using these cells in the treatment or prevention of T-cell alloreactivity in hematopoietic or solid organ allogeneic transplantation.Abstract:
Background Bone marrow mesenchymal stem cells (MSCs) are currently being investigated in preclinical and clinical settings because of their multipotent differentiative capacity or, alternatively, their immunosuppressive function. The aim of this study was to evaluate dental pulp (DP) as a potential source of MSCs instead of bone marrow (BM). Methods Flow cytometric analysis showed that DP-MSCs and BM-MSCs were equally SH2, SH3, SH4, CD29 and CD 166 positive. The in vitro proliferative kinetics of MSCs were measured by 3H-thymidine incorporation uptake. The immunosuppressive function of MSCs was then tested by coculturing PHA-stimulated allogeneic T cells with or without MSCs for 3 days. Results BM-MSCs could be differentiated in vitro into osteogenic, chondrogenic and adipogenic lineages. DP-MSCs showed osteogenic and adipocytic differentiation, but did not differentiate into chondrocytes. Although DP-MSCs grow rapidly in vitro between day 3 and day 8 of culture and then decrease their proliferation by day 15, BM-MSCs have a stable and continuous proliferation over the same period of time. The addition of DP-MSCs or BM-MSCs resulted in 91 +/- 4% and 75 +/- 3% inhibition of T cell response, respectively, assessed by a 3H-thymidine assay. Conclusions Dental pulp is an easily accessible and efficient source of MSCs, with different kinetics and differentiation potentialities from MSCs as isolated from the bone marrow. The rapid proliferative capacity together with the immunoregulatory characteristics of DP-MSCs may prompt future studies aimed at using these cells in the treatment or prevention of T-cell alloreactivity in hematopoietic or solid organ allogeneic transplantation.read more
Citations
More filters
Journal ArticleDOI
Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine
TL;DR: This article will review the isolation and characterization of the properties of different dental MSC-like populations in comparison with those of other MSCs, such as BMMSCs.
Journal ArticleDOI
PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages.
Felicia Soo Lee Ng,Shayne Boucher,Susie Koh,Konduru S. R. Sastry,Lucas G. Chase,Uma Lakshmipathy,Cleo Choong,Zheng Yang,Mohan C. Vemuri,Mahendra S. Rao,Vivek Tanavde +10 more
TL;DR: This study illustrates it is possible to predict signaling pathways active in cellular differentiation and growth using microarray data and experimentally verify these predictions.
Journal ArticleDOI
Immune Properties of Human Umbilical Cord Wharton's Jelly‐Derived Cells
Mark L. Weiss,Cameron Anderson,Satish Medicetty,Kiran Seshareddy,Rita Weiss,Irene Vanderwerff,Deryl L. Troyer,Kevin R. Mcintosh +7 more
TL;DR: The results indicate that human UCMS cells inhibit splenocyte proliferation response to concanavalin A stimulation, that they do not stimulate T‐cell proliferation in a one‐way MLR, and that they inhibit the proliferation of stimulated T cells in a two‐ way MLR.
Journal ArticleDOI
Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers
Irina Kerkis,Alexandre Kerkis,D. Dozortsev,Gaëlle Chopin Stukart-Parsons,Silvia Maria Gomes Massironi,Lygia da Veiga Pereira,Arnold I. Caplan,Humberto F. Cerruti +7 more
TL;DR: In vivo transplantation of immature dental pulp stem cells into immunocompromised mice, they showed dense engraftment in various tissues and the relative ease of recovery and the expression profiles of various markers justify further exploration of IDPSC for clinical therapy.
Journal ArticleDOI
Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes.
Riccardo D'aquino,Alfredo De Rosa,Vladimiro Lanza,Virginia Tirino,Luigi Laino,Antonio Graziano,Vincenzo Desiderio,Gregorio Laino,Gianpaolo Papaccio +8 more
TL;DR: It is demonstrated that a DPC/collagen sponge biocomplex can completely restore human mandible bone defects and indicates that this cell population could be used for the repair and/or regeneration of tissues and organs.
References
More filters
Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues
TL;DR: Marrow stromal cells present an intriguing model for examining the differentiation of stem cells and have several characteristics that make them potentially useful for cell and gene therapy.
Journal ArticleDOI
Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
TL;DR: Postnatal human DPSCs are isolated that have the ability to form a dentin/pulp-like complex and are compared with human bone marrow stromal cells, known precursors of osteoblasts.
Journal ArticleDOI
Mesenchymal Stem Cells
TL;DR: The bone marrow contains multipotent MSC, which can be easily isolated and cultured in vitro, and the possibility of their clinical use in cell and gene therapy is analyzed.
Journal ArticleDOI
Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli
Massimo Di Nicola,Carmelo Carlo-Stella,Michele Magni,Marco Milanesi,Paolo Longoni,Paola Matteucci,Salvatore Grisanti,Alessandro M. Gianni +7 more
TL;DR: The data demonstrate that autologous or allogeneic BMSCs strongly suppress T-lymphocyte proliferation, this phenomenon that is triggered by both cellular as well as nonspecific mitogenic stimuli has no immunologic restriction, and T-cell inhibition is not due to induction of apoptosis and is likely due to the production of soluble factors.