Journal ArticleDOI
Muscarinic cholinergic receptor-mediated modulation on striatal c-fos mRNA expression induced by levodopa in rat brain.
TLDR
It is suggested that the muscarinic cholinergic system may modulate the levodopa-induced c-fos mRNA expression which then regulates the expression of other mRNAs.Abstract:
To clarify the interactions between dopamine receptors and muscarinic cholinergic receptors by which neurotransmitters may affect genetic responses, we studied the effects of the muscarinic cholinergic agonist, carbachol, and the muscarinic cholinergic antagonist, trihexyphenidyl, on levodopa-induced c-fos messenger RNA (mRNA) expression in rat striatum. Animals were administered levodopa (levodopa with one-tenth dosage of carbidopa), carbachol or thrihexyphenidyl alone or administered in combination as levodopa (100 mg/kg) + carbachol, or levodopa + trihexyphenidyl given as a single bolus. Levodopa given alone increase the expression of c-fos mRNA. Although carbachol or trihexyphenidyl alone was ineffective in inducing c-fos mRNA, the combination of levodopa and carbachol (⩾ 0.1 mg/kg) significantly suppressed the induction of c-fos mRNA as compared with levodopa given alone. The combined administration of levodopa and trihexyphenidyl showed a trend toward an additive effect on the induction of c-fos mRNA vs levodopa alone. These findings suggest that the muscarinic cholinergic system may modulate the levodopa-induced c-fos mRNA expression which then regulates the expression of other mRNAs.read more
Citations
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Journal ArticleDOI
Muscarinic receptors regulate striatal neuropeptide gene expression in normal and amphetamine-treated rats.
J.Q. Wang,Jacqueline F. McGinty +1 more
TL;DR: The concept that cholinergic transmission, via interaction with muscarinic receptors, inhibits basal and D1 receptor-stimulated striatonigral dynorphin/substance P gene expression and facilitates striatopallidal enkephalin gene expression is supported.
Journal ArticleDOI
Scopolamine augments C-fos and zif/268 messenger rna expression induced by the full D1 dopamine receptor agonist SKF-82958 in the intact rat striatum
J.Q. Wang,Jacqueline F. McGinty +1 more
TL;DR: Ability of SKF-82958 to induce immediate early gene messenger RNA expression in normosensitive dorsal and ventral striatum of the rat is demonstrated and intrinsic muscarinic receptor-mediated cholinergic transmission in the striatum may provide an activity-dependent inhibitory control on striatal D(1) receptor stimulation.
Journal ArticleDOI
Intrastriatal injection of a muscarinic receptor agonist and antagonist regulates striatal neuropeptide mRNA expression in normal and amphetamine-treated rats
TL;DR: Data demonstrate an inhibition of striatonigral, and facilitation of striatopallidal, gene expression through activation of local striatal muscarinic receptors, which is consistent with the changes seen after systemic administration ofMuscarinic agents.
Journal ArticleDOI
Differential expression of immediate early genes in distinct layers of rat cerebral cortex after selective immunolesion of the forebrain cholinergic system
TL;DR: Whether reduction or loss of cortical cholinergic activity results in any particular change in the expression of the proto‐on‐cogenes c‐fos and/or c‐jun is shown to be unclear.
Journal ArticleDOI
Pitfalls in Assessment of c-fos mRNA Expression in the Brain: Effects of Animal Handling
Masato Asanuma,Norio Ogawa +1 more
TL;DR: The results suggest that c-fos mRNA induction after a single stimulation might be affected by the types or intensities of animal handling and that effects of animalhandling must be considered when estimating c- fos RNA induction.
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