N6-methyl-adenine: an epigenetic signal for DNA-protein interactions.
Didier Wion,Josep Casadesús +1 more
TLDR
N6-methyl-adenine is found in the genomes of bacteria, archaea, protists and fungi and has a role in Brucella abortus infection and α-proteobacteria.Abstract:
N(6)-methyl-adenine is found in the genomes of bacteria, archaea, protists and fungi. Most bacterial DNA adenine methyltransferases are part of restriction-modification systems. Certain groups of Proteobacteria also harbour solitary DNA adenine methyltransferases that provide signals for DNA-protein interactions. In gamma-proteobacteria, Dam methylation regulates chromosome replication, nucleoid segregation, DNA repair, transposition of insertion elements and transcription of specific genes. In Salmonella, Haemophilus, Yersinia and Vibrio species and in pathogenic Escherichia coli, Dam methylation is required for virulence. In alpha-proteobacteria, CcrM methylation regulates the cell cycle in Caulobacter, Rhizobium and Agrobacterium, and has a role in Brucella abortus infection.read more
Citations
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Journal ArticleDOI
Function and information content of DNA methylation
TL;DR: These observations indicate that the underlying DNA sequence largely accounts for local patterns of methylation, which is highly informative when studying gene regulation in normal and diseased cells, and it can potentially function as a biomarker.
Journal ArticleDOI
Opportunities and challenges in long-read sequencing data analysis.
Shanika L. Amarasinghe,Shanika L. Amarasinghe,Shian Su,Shian Su,Xueyi Dong,Xueyi Dong,Luke Zappia,Matthew E. Ritchie,Matthew E. Ritchie,Quentin Gouil,Quentin Gouil +10 more
TL;DR: The current landscape of available tools is reviewed, the principles of error correction, base modification detection, and long-read transcriptomics analysis are focused on, and the challenges that remain are highlighted.
Journal ArticleDOI
Epigenetic Gene Regulation in the Bacterial World
Josep Casadesús,David A. Low +1 more
TL;DR: DNA adenine methylation plays roles in the virulence of diverse pathogens of humans and livestock animals, including pathogenic Escherichia coli, Salmonella, Vibrio, Yersinia, Haemophilus, and Brucella.
Journal ArticleDOI
DNA Methylation on N6-Adenine in C. elegans
Eric L. Greer,Eric L. Greer,Mario Andres Blanco,Mario Andres Blanco,Lei Gu,Lei Gu,Erdem Sendinc,Erdem Sendinc,Jianzhao Liu,David Aristizábal-Corrales,David Aristizábal-Corrales,Chih-Hung Hsu,Chih-Hung Hsu,L. Aravind,Chuan He,Yang Shi,Yang Shi +16 more
TL;DR: The presence of adenine N(6)-methylation (6mA) in C. elegans DNA is demonstrated and the exciting possibility that 6mA may be a carrier of heritable epigenetic information in eukaryotes is raised.
Journal ArticleDOI
N6-methyladenine DNA modification in Drosophila.
Guoqiang Zhang,Hua Huang,Di Liu,Ying Cheng,Xiaoling Liu,Wenxin Zhang,Ruichuan Yin,Dapeng Zhang,Peng Zhang,Jianzhao Liu,Chaoyi Li,Baodong Liu,Yuewan Luo,Yuanxiang Zhu,Ning Zhang,Shunmin He,Chuan He,Hailin Wang,Dahua Chen +18 more
TL;DR: It is shown that 6mA is present in Drosophila genome and that the 6mA modification is dynamic and is regulated by the Dosophila Tet homolog, DNA 6mA demethylase (DMAD), during embryogenesis, and biochemical assays demonstrate that DMAD directly catalyzes 6AMA demethylation in vitro.
References
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