Journal ArticleDOI
Nano-localized single-cell nano-electroporation.
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TLDR
The platform successfully delivers dyes, QDs, and plasmids into different cell types with the variation of field strength, pulse duration, and the number of pulses, which is potentially beneficial for cellular therapy and diagnostic purposes.Abstract:
The ability to deliver foreign cargos into single living cells is of great interest in cell biology and therapeutic research. Here, we have reported a single or multiple position based nano-localized single-cell nano-electroporation platform. The device consists of an array of triangular shape ITO nano-electrodes with a 70 nm gap between two nano-electrodes, each having a 40 nm tip diameter. The voltage is applied between nano-electrodes to generate an intense electric field, which electroporates multiple nano-localized regions of the targeted single-cell membrane, and biomolecules are gently delivered into cells by pressurizing pump flow, without affecting cell viability. The platform successfully delivers dyes, QDs, and plasmids into different cell types with the variation of field strength, pulse duration, and the number of pulses. This new approach allows us to analyze delivery of different biomolecules into single living cells with high transfection efficiency (>96%, for CL1-0 cells) and high cell viability (∼98%), which are potentially beneficial for cellular therapy and diagnostic purposes.read more
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Journal ArticleDOI
Microfluidic mechanoporation for cellular delivery and analysis
TL;DR: A brief review of the most recent developments on microfluidic-based mechanoporation strategies such as microinjection, nanoneedle arrays, cell-squeezing, and hydroporation techniques with their working principle, device fabrication, cellular delivery, and analysis is provided in this paper .
Journal ArticleDOI
Pulsed laser assisted high-throughput intracellular delivery in hanging drop based three dimensional cancer spheroids.
Pallavi Gupta,Srabani Kar,Ashish Kumar,Fan-Gang Tseng,Shantanu Pradhan,Pallab Sinha Mahapatra,Tuhin Subhra Santra +6 more
TL;DR: In this article, a high-throughput intracellular delivery of a model fluorescent cargo (propidium iodide, PI) within 3D SiHa human cervical cancer spheroids was demonstrated.
Journal ArticleDOI
Advances in microfluidic strategies for single-cell research
TL;DR: A review of microfluidic strategies for single-cell research relevant to tumorigenesis and evolution, immune responses, and drug discovery can be found in this article , where the authors discuss the design of microfluidic devices for singlecell analysis, the current methods of micro-fluidIC devices for individual cell isolation, and applications of microFluidics-based single-cells analysis in cell biology, disease diagnosis, and therapy.
Journal ArticleDOI
Approaches and materials for endocytosis-independent intracellular delivery of proteins.
TL;DR: A review of the latest advances in direct intracellular delivery of proteins through membrane perforation, membrane translocation, and membrane fusion was summarized in this article , and the mechanisms, related materials and potential therapeutic in living cells/in vivo for each approach were discussed in detail, and the future development in this promising field was briefly presented.
Journal ArticleDOI
Can titanium oxide nanotubes facilitate intracellular delivery by laser-assisted photoporation?
L. Mohan,Srabani Kar,Srabani Kar,Ren Hattori,Miho Ishii-Teshima,Parthasarathi Bera,Sounak Roy,Tuhin Subhra Santra,Takayuki Shibata,Moeto Nagai +9 more
TL;DR: In this article, a nanosecond pulse laser-assisted photoporation using titanium-oxide nanotubes (TNT) for highly efficient intracellular delivery has been established.
References
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Journal ArticleDOI
Theory of electroporation: A review
TL;DR: This review of the essential features of theoretical models of electroporation focuses on transient aqueous pore models, which can account for key features of mechanical instability and dramatic reversible electrical behavior of certain planar membranes and of cell membranes, and some features of molecular transport.
Journal ArticleDOI
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Yuhong Xu,Francis C. Szoka +1 more
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