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Synthetic DNA delivery systems.
Dan Luo,Saltzman Wm +1 more
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TLDR
The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology and rapid advances have recently been made in understanding of mechanisms for DNA stability and transport within cells.Abstract:
The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. Indeed, most current systems address only one of the obstacles to DNA delivery by enhancing DNA uptake. In fact, the effectiveness of gene expression is also dependent on several additional factors, including the release of intracellular DNA, stability of DNA in the cytoplasm, unpackaging of the DNA-vector complex, and the targeting of DNA to the nucleus. Delivery systems of the future must fully accommodate all these processes to effectively shepherd DNA across the plasma membrane, through the hostile intracellular environment, and into the nucleus.read more
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Principles of nanoparticle design for overcoming biological barriers to drug delivery
TL;DR: By successively addressing each of the biological barriers that a particle encounters upon intravenous administration, innovative design features can be rationally incorporated that will create a new generation of nanotherapeutics, realizing a paradigmatic shift in nanoparticle-based drug delivery.
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Designing materials for biology and medicine
Robert Langer,David A. Tirrell +1 more
TL;DR: New challenges and directions in biomaterials research are discussed, including synthetic replacements for biological tissues, designing materials for specific medical applications, and materials for new applications such as diagnostics and array technologies.
Journal ArticleDOI
Polymeric system for dual growth factor delivery
TL;DR: This is the first report of a vehicle capable of delivery of multiple angiogenic factors with distinct kinetics, and these results clearly indicate the importance of multiple growth factor action in tissue regeneration and engineering.
Journal ArticleDOI
Small-scale systems for in vivo drug delivery.
TL;DR: Micro- and nanotechnologies are enabling the design of novel methods such as radio-frequency addressing of individual molecules or the suppression of immune response to a release device, but current challenges include the need to balance the small scale of the devices with the quantities of drugs that are clinically necessary.
Journal ArticleDOI
A peptide carrier for the delivery of biologically active proteins into mammalian cells.
TL;DR: A new strategy for protein delivery based on a short amphipathic peptide carrier, Pep-1, which is able to efficiently deliver a variety of peptides and proteins into several cell lines in a fully biologically active form, without the need for prior chemical covalent coupling or denaturation steps.
References
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Journal ArticleDOI
A new technique for the assay of infectivity of human adenovirus 5 DNA.
Frank L. Graham,A. J. Van Der Eb +1 more
TL;DR: A new technique for assaying infectivity of adenovirus 5 DNA has been developed and a reproducible relationship between amounts of DNA inoculated per culture and numbers of plaques produced was demonstrated.
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A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine
Otmane Boussif,Frank Lezoualc'h,Maria Antonietta Zanta,Mojgan Mergny,Daniel Scherman,Barbara A. Demeneix,Jean-Paul Behr +6 more
TL;DR: Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
Journal ArticleDOI
Direct gene transfer into mouse muscle in vivo.
Jon A. Wolff,Robert W. Malone,Phillip Williams,Wang Chong,Gyula Acsadi,Agnes Jani,Philip L. Felgner +6 more
TL;DR: RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo and expression was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions.
Journal ArticleDOI
Gene transfer into mouse lyoma cells by electroporation in high electric fields.
TL;DR: A simple physical model for the enhanced DNA penetration into cells in high electric fields is proposed, according to which the interaction of the external electric field with the lipid dipoles of a pore configuration induces and stabilizes the permeation sites and thus enhances cross membrane transport.