Native Mass Spectrometry: What is in the Name?
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TLDR
The origins of and (re)define the term native MS are described, portraying in detail what the authors meant by “native MS,” when the term was coined and also describing what it does (according to us) not entail.About:
This article is published in Journal of the American Society for Mass Spectrometry.The article was published on 2017-01-01 and is currently open access. It has received 415 citations till now.read more
Citations
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Emerging and Re-Emerging Warheads for Targeted Covalent Inhibitors: Applications in Medicinal Chemistry and Chemical Biology
Matthias Gehringer,Stefan Laufer +1 more
TL;DR: An overview of warheads-beyond α,β-unsaturated amides-recently used in the design of targeted covalent ligands is provided, with special emphasis on the discussion of reactivity and of case studies illustrating applications in medicinal chemistry and chemical biology.
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Ambient ionization Mass Spectrometry: Recent Developments and Applications
TL;DR: In the last 15 years, innovations in the field of ambient ionization MS have grown expansively, pushing these technologies far past their point of conception and integrating them into the broader scientific community in creative and stimulating ways.
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Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.
Daniel P. Donnelly,Catherine M. Rawlins,Caroline J. DeHart,Luca Fornelli,Luis F. Schachner,Ziqing Lin,Jennifer L. Lippens,Krishna Aluri,Krishna Aluri,Richa Sarin,Richa Sarin,Bifan Chen,Carter Lantz,Wonhyeuk Jung,Kendall Johnson,Antonius Koller,Jeremy J. Wolff,Iain D. G. Campuzano,Jared R. Auclair,Alexander R. Ivanov,Julian P. Whitelegge,Ljiljana Paša-Tolić,Julia Chamot-Rooke,Paul O. Danis,Lloyd M. Smith,Yury O. Tsybin,Joseph A. Loo,Ying Ge,Neil L. Kelleher,Jeffrey N. Agar +29 more
TL;DR: The Consortium for Top-Down Proteomics presents a decision-tree-based guide to sample preparation and analysis protocols for researchers performing top-down mass-spectrometry-based analysis of intact proteins.
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Ion Activation Methods for Peptides and Proteins
TL;DR: An array of activation methods has been developed to energize ions and promote reproducible fragmentation in a way to cause informative dissociation of the ions of interest.
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Addressing a Common Misconception: Ammonium Acetate as Neutral pH “Buffer” for Native Electrospray Mass Spectrometry
TL;DR: This work concludes that neutral ammonium acetate solutions electrosprayed in positive ion mode will likely undergo acidification down to pH 4.75 ± 1 in the ESI plume, and it is hoped that the habit of referring to pH 7 solutions as ammoniumacetate “buffer” will disappear from the literature.
References
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Electrospray ionization for mass spectrometry of large biomolecules
TL;DR: Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.
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Guillaume Rigaut,Anna Shevchenko,Berthold Rutz,Matthias Wilm,Matthias Mann,Bertrand Séraphin +5 more
TL;DR: A generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag and Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein.
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Analytical properties of the nanoelectrospray ion source.
Matthias Wilm,Matthias Mann +1 more
TL;DR: Improved desolvation in nanoES led to instrument-limited resolution of the signals of a glycoprotein and the ability to signal average extensively allowed the C-terminal sequencing of a 40 kDa protein.
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The BioPlex Network: A Systematic Exploration of the Human Interactome
Edward L. Huttlin,Lily Ting,Raphael J. Bruckner,Fana Gebreab,Melanie P. Gygi,John Szpyt,Stanley Tam,Gabriela Zarraga,Greg Colby,Kurt Baltier,Rui Dong,Virginia Guarani,Laura Pontano Vaites,Alban Ordureau,Ramin Rad,Brian K. Erickson,Martin Wühr,Joel M. Chick,Bo Zhai,Deepak Kolippakkam,Julian Mintseris,Robert A. Obar,Robert A. Obar,Tim Harris,Spyros Artavanis-Tsakonas,Spyros Artavanis-Tsakonas,Mathew E. Sowa,Pietro De Camilli,Joao A. Paulo,J. Wade Harper,Steven P. Gygi +30 more
TL;DR: Using high-throughput affinity-purification mass spectrometry, BioPlex is used to identify interacting partners for 2,594 human proteins in HEK293T cells and reveals associations among thousands of protein domains, suggesting a basis for examining structurally related proteins.