Nitrogen Source Regulates Glutamate Dehydrogenase NADP Synthesis in Neurospora crassa
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TLDR
Quantitative immunoelectrophoresis established that, under all conditions, enzyme activity corresponded to enzyme concentration, and isotope incorporation studies demonstrated that the nitrogen source exerts its regulation at the level of de novo enzyme synthesis.Abstract:
Neurospora crassa glutamate dehydrogenase-NADP (EC 1.3.1.3) has a higher activity when mycelium is grown on ammonium or nitrate as nitrogen source than when grown on glutamate or glutamine. Quantitative immunoelectrophoresis established that, under all conditions, enzyme activity corresponded to enzyme concentration. Isotope incorporation studies demonstrated that the nitrogen source exerts its regulation at the level of de novo enzyme synthesis.read more
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Ammonium assimilation by spruce—Hebeloma sp. ectomycorrhizas
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Nitrogen assimilating enzymes in the white button mushroom Agaricus bisporus
Johan J. P. Baars,H.J.M. op den Camp,J. M. H. Hermans,V. Mikes,C. van der Drift,L.J.L.D. van Griensven,Godfried D. Vogels +6 more
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The involvement of glutamate dehydrogenase and glutamine synthetase in ammonia assimilation by the rapidly growing ectomycorrhizal ascomycete, Cenococcum geophilum Fr.
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Glutamine metabolism and cycling in Neurospora crassa.
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References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
Journal ArticleDOI
The occurrence of two different glutamic dehydrogenases in Neurospora.
B.D. Sanwal,Madhu Lata +1 more
TL;DR: Many wild-type strains of Neurospora crassa produce two glutamic dehydrogenases, one specific for triphosphopyridine nucleotide and another for diphosphopyrsine nucleotide, which may be reasons for the presence of two different enzymes catalyzing the same reaction.