Journal ArticleDOI
Nucleotide Sequence of the Gene Coding for the Bacteriophage MS2 Coat Protein
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TLDR
By characterization of fragments, isolated from a nuclease digest of MS2 RNA, the entire nucleotide sequence of the coat gene was established and a “flower”-like model is proposed for the secondary structure.Abstract:
By characterization of fragments, isolated from a nuclease digest of MS2 RNA, the entire nucleotide sequence of the coat gene was established. A “flower”-like model is proposed for the secondary structure. The genetic code makes use of 49 different codons to specify the sequence of the 129 amino-acids long coat polypeptide.read more
Citations
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The 3′-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites
John Shine,Lynn Dalgarno +1 more
TL;DR: It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons.
Journal ArticleDOI
Improved Estimation of Secondary Structure in Ribonucleic Acids
Ignacio Tinoco,Philip N. Borer,Barbara Dengler,Mark D. Levin,Olke C. Uhlenbeck,Donald M. Crothers,J. Bralla +6 more
TL;DR: This method can be used for predicting and assessing possible secondary structures for recently determined RNA sequences and new experimental and theoretical results allow us to improve the method, without making it more complicated.
Journal ArticleDOI
Rapid evolution of RNA genomes
John J. Holland,Katherine R. Spindler,Katherine R. Spindler,Frank M. Horodyski,Elizabeth A. Grabau,Stuart T. Nichol,Scott VandePol +6 more
TL;DR: RNA viruses show high mutation frequencies partly because of a lack of the proofreading enzymes that assure fidelity of DNA replication, and high rates of replication reflected in rates of RNA genome evolution which can be more than a millionfold greater than the rates of the DNA chromosome evolution of their hosts.
Journal ArticleDOI
Determinant of cistron specificity in bacterial ribosomes
John Shine,Lynn Dalgarno +1 more
TL;DR: Complementarity relationships between this sequence and a purine-rich tract in the ribosome binding site of different bacterial mRNAs suggest that the 3′-end of 16S RNA determines the intrinsic capacity of ribosomes to translate a particular cistron.
Journal ArticleDOI
The sequence of sequencers: The history of sequencing DNA.
TL;DR: This article traverses those years, iterating through the different generations of sequencing technology, highlighting some of the key discoveries, researchers, and sequences along the way.
References
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Journal ArticleDOI
A two-dimensional fractionation procedure for radioactive nucleotides
TL;DR: High-voltage ionophoresis is used in both dimensions for the two-dimensional fractionation of ribonuclease digests of 32P-labelled RNA and the determination of the sequence of a nucleotide by partial digestion with spleen phosphodiesterase.
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Electrophoretic separation of viral nucleic acids on polyacrylamide gels
TL;DR: The elution of viral RNA from gel slices and the demonstration of infectivity after electrophoresis are described, showing a general relationship between the logarithm of the molecular weight and the relative electrophoretic mobility.
Journal ArticleDOI
Estimation of Secondary Structure in Ribonucleic Acids
TL;DR: A simple method for estimating the secondary structure of an RNA molecule has been proposed on the basis of the knowledge of its sequence.
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Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA
TL;DR: The initiator regions of the three cistrons of R17 bacteriophage RNA have been isolated and sequenced and contain a UGA triplet as well as the expected AUG and two contain the sequence GGUUUGA.
Journal ArticleDOI
Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
George G. Brownlee,F. Sanger +1 more
TL;DR: A two-dimensional fractionation procedure has been developed for separating radioactively-labelled oligonucleotides of up to 50 residues long, using uniformly 32P-labelling 5S RNA of Escherichia coli as a model compound.