One-Step Sample Concentration, Purification, and Albumin Depletion Method for Urinary Proteomics
TLDR
A one-step sample preparation workflow that simultaneously concentrates proteins, purifies by removing salts and other low molecular weight compounds, and depletes (albumin) from urine samples is developed that can be multiplexed and compatible with a diverse range of downstream multidimensional separation technologies.Abstract:
Workflows in urinary proteomics studies are often complex and require many steps to enrich, purify, deplete, and separate the complex mixture. Many of these methods are laborious, are time-consuming, and have the potential for error. Although individual steps of these methods have been previously studied, their downstream compatibilities with fractionation technologies such as off-gel electrophoresis have not been investigated. We developed a one-step sample preparation workflow that simultaneously (i) concentrates proteins, (ii) purifies by removing salts and other low molecular weight compounds, and (iii) depletes (albumin) from urine samples. This simple and robust workflow can be multiplexed and is compatible with a diverse range of downstream multidimensional separation technologies. Additionally, because of its high reproducibility and flexibility in processing samples with different volumes and concentrations, it has the potential to be used for standardization of urinary proteomics studies, as well as for studying other body fluids of similar complexity.read more
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Physalis angulata leaf extract ameliorates L-N G -nitroarginine methyl ester (L-NAME)-induced preeclampsia symptoms in rats through improved endothelial progenitor cells and endothelial cells due to reduced antiangiogenic factor and oxidative stress [version 1; peer review: awaiting peer review]
Dian Nugrahenny,Achmad Rudijanto,Nur Hidayati Ika Permatasari,I Wayan Arsana Wiyasa,M. Aris Widodo,Karyono Mintaroem,Edi Widjajanto,Mustofa Mustofa +7 more
TL;DR: Physalis angulata leaf extract reduces antiangiogenic factor and oxidative stress and enhances eNOS/NO signaling and reverses L-NAME-induced hypertension and proteinuria in preeclampsia rats by improving EPC and endothelial cell function.
Journal ArticleDOI
Physalis angulata leaf extract ameliorates L-NG-nitroarginine methyl ester (L-NAME)-induced preeclampsia symptoms in rats through improved endothelial progenitor cells and endothelial cells due to reduced antiangiogenic factor and oxidative stress
Dian Nugrahenny,Achmad Rudijanto,Nur Hidayati Ika Permatasari,I Wayan Arsana Wiyasa,M. Aris Widodo,Karyono Mintaroem,Edi Widjajanto,Mustofa Mustofa +7 more
TL;DR: Physalis angulata leaf extract improves EPC and endothelial cell function and reverses L-NAME-induced hypertension and proteinuria in preeclampsia rats and enhances eNOS/NO signaling.
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TL;DR: A significant number of proteins to be over-expressed in the urine from INS patients, which includes a high percentage of glycosylated proteins, strongly suggests correlation with the neuronal system disorders network, specifically acute fatigue.
An ion mobility enabled data independent multi-omics approach to quantitatively characterize urine from children diagnosed with idiopathic nephrotic syndrome
Lee A. Gethings,Johannes P. C. Vissers,John P. Shockcor,Stephen McDonald,Sandra Kraljević Pavelić,Mirela Sedić,Maja Lemac,Danica Batinić,James Ian Langridge +8 more
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