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Journal ArticleDOI

Optimization of batch fermentation conditions for dextran production

TLDR
The nutrient medium, temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F) and the optimal values of tested variables were found to be sucrose, yeast extract and K2HPO4.
Abstract
The nutrient medium (containing sucrose, yeast extract and K2HPO4), temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F). A 25−1 fractional factorial central composite experimental design was attempted. Multistage Monte Carlo optimization program was used to maximize the multiple regression equation obtained. The optimal values of tested variables for maximal dextran production were found to be: sucrose, 300 g/l; yeast extract, 10 g/l; K2HPO4, 30 g/l; temperature, 23°C and initial pH 8.3 with a predicted dextran yield of 154 g/l.

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Journal ArticleDOI

Karakteristik Isolat Bakteri Penghasil Dekstran dari Batang Tebu (Saccharum officinarum L.)

TL;DR: Based on the results of this study, the Paenibacillus polymyxa TBT 3.2 can be used as a dextran producing bacteria.
Dissertation

Optimization of enzyme extractions for total folate in cereals and determination of total folate in breakfast cereals and snack foods

Sungeun Cho
TL;DR: The trienzyme digestion including protease, α-amylase, and conjugase for the extraction of total folate from cereals was optimized using response surface methodology.
Journal ArticleDOI

Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells

TL;DR: Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation to improve the concentration to 1350 μg/ml higher than that produced from free cells cultures and could be maintained for longer than 17 days in a repeated batch system.

Optimization of Some Culture Conditions to Enhance -Amylase Production using Response Surface Methodology

TL;DR: Amylases of microorganisms have a broad spectrum of industrial applications as they are more stable than when prepared with plant and animal amylases, and the economical bulk production capacity and the fact that microbes are easy is the major advantage.
Journal ArticleDOI

Modeling and Optimizing of Mechanically Agitated Vessels by Central Composite Rotatable Design Method

TL;DR: In this article, a central composite rotatable design (CCRD) methodology was used to analyze the effect of some operating variables on gas-liquid two phase mixing time in an agitated tank driven by dual 6-blade Rushton turbines.
References
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Book

Encyclopedia of polymer science and technology

TL;DR: In this article, the authors present a survey of the properties of polymers and their application in the field of chemical engineering, including the following: Coextrusion, Injection Molding, Flexible Packaging, Fibers, Polymer-Clay, and Plasticizers.
Journal ArticleDOI

The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides.

TL;DR: Observation on factors affecting production of dextransucrase from Leuconostoc mesenteroides, strain NRRL B-512 and the possibility that more than one enzyme may be involved in the synthesis ofdextran is recognized.
Journal ArticleDOI

Medium development for xanthan production

TL;DR: Mineral sources such as CaCO3 and KH2PO4 proved to be important factors influencing polysaccharide production and quality and the use of high carbon to nitrogen ratios in the defined medium resulted in a major increase in the broth consistency index.
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