Journal ArticleDOI
Opto-electronic immunosensors: a review of optical immunoassay at continuous surfaces.
TLDR
Overall, it was considered that the IRS systems and ellipsometric approaches offered the most promise for the design of a specific immunosensor device.About:
This article is published in Biosensors.The article was published on 1985-01-01. It has received 116 citations till now. The article focuses on the topics: Attenuated total reflection & Light scattering.read more
Citations
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Journal ArticleDOI
Total internal reflection fluorescence
TL;DR: TIRF is an optical effect particularly well-suited to the study of molecular and cellular phenomena at liquid/solid interfaces that is central to a wide range of biochemical and biophysical processes.
Journal ArticleDOI
A Decade of Development in Immunoassay Methodology
TL;DR: The use of monoclonal antibodies is now widespread, and the methodologies of labels and of solid-phase components are much more sophisticated; new assay formulations, novel homogeneous systems, immunosensors, free-analyte assays, the importance of thorough validation and of interfering substances, and future trends are discussed.
BookDOI
Principles And Practice Of Immunoassay
TL;DR: The antigen-antibody reaction production of polyclonal antibodies production of monoclonal antibodies modification of antibodies separation techniques for heterogeneous immunoassays properties of antibodies and general considerations on reagnet optimization are considered.
Journal ArticleDOI
Capacitive monitoring of protein immobilization and antigen–antibody reactions on monomolecular alkylthiol films on gold electrodes
TL;DR: The N-hydroxysuccinimide and carbodiimide methods were identified as most suitable for protein immobilization in that they did not compromise the insulating properties of the alkylthiol layer and led to maximal increase of its dielectric thickness.
Journal ArticleDOI
Performance of a highly sensitive optical waveguide Mach-Zehnder interferometer immunosensor
TL;DR: In this paper, a planar optical waveguide is used to obtain a steep dependence of the propagation velocity on the refractive-index profile near the surface, which is measured interferometrically using a Mach-Zehnder interferometer set-up.
References
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Journal ArticleDOI
Immunoassay of Endogenous Plasma Insulin in Man
TL;DR: For years investigators have sought an assay for insulin which would combine virtually absolute specificity with a high degree of sensitivity, sufficiently exquisite for measurement of the minute insulin concentrations usually present in the circulation as mentioned in this paper.
Journal ArticleDOI
Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion
G. Köhler,C. Milstein +1 more
TL;DR: Cell fusion techniques have been used to produce hybrids between myeloma cells and antibody‐producing cells that are permanently adapted to grow in tissue culture and are capable of inducing antibody-producing tumors in mice.
Journal ArticleDOI
Built-Up Films of Barium Stearate and Their Optical Properties
TL;DR: The technique of depositing successive single layers of molecules of various stearates on a solid surface is described in this paper, where a photograph shows films built in a series of steps having intervals of 2 molecular layers.
Journal ArticleDOI
Antibodies to bradykinin and angiotensin: a use of carbodiimides in immunology.
TL;DR: Antibodies to bradykinin and angiotensin have been produced in rabbits by the use of conjugates containing albumin and the hapten, covalently bound using water-soluble carbodiimide reagents.
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Kinetics of antibody-binding to surface-immobilized antigen: Influence of mass transport on the enzyme-linked immunosorbent assay (ELISA)
Håkan Nygren,Manne Stenberg +1 more