Oxidative stress increased respiration and generation of reactive oxygen species, resulting in ATP depletion, opening of mitochondrial permeability transition, and programmed cell death.
Reads0
Chats0
TLDR
The role of the mitochondria in the generation of oxidative burst and induction of programmed cell death in response to brief or continuous oxidative stress in Arabidopsis cells is analyzed and it is suggested that protease activation is a necessary step in the cell death pathway after mitochondrial damage.Abstract:
Mitochondria constitute a major source of reactive oxygen species and have been proposed to integrate the cellular responses to stress. In animals, it was shown that mitochondria can trigger apoptosis from diverse stimuli through the opening of MTP, which allows the release of the apoptosis-inducing factor and translocation of cytochrome c into the cytosol. Here, we analyzed the role of the mitochondria in the generation of oxidative burst and induction of programmed cell death in response to brief or continuous oxidative stress in Arabidopsis cells. Oxidative stress increased mitochondrial electron transport, resulting in amplification of H(2)O(2) production, depletion of ATP, and cell death. The increased generation of H(2)O(2) also caused the opening of the MTP and the release of cytochrome c from mitochondria. The release of cytochrome c and cell death were prevented by a serine/cysteine protease inhibitor, Pefablock. However, addition of inhibitor only partially inhibited the H(2)O(2) amplification and the MTP opening, suggesting that protease activation is a necessary step in the cell death pathway after mitochondrial damage.read more
Citations
More filters
Journal ArticleDOI
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction
Klaus Apel,Heribert Hirt +1 more
TL;DR: The mechanisms of ROS generation and removal in plants during development and under biotic and abiotic stress conditions are described and the possible functions and mechanisms for ROS sensing and signaling in plants are compared with those in animals and yeast.
Journal ArticleDOI
Drought tolerance improvement in crop plants: An integrated view from breeding to genomics
Luigi Cattivelli,Fulvia Rizza,Franz-W. Badeck,Elisabetta Mazzucotelli,Anna M. Mastrangelo,Enrico Francia,Caterina Marè,Alessandro Tondelli,A. Michele Stanca +8 more
TL;DR: Breeders are asked to blend together all knowledge on the traits sustaining yield under drought and to accumulate the most effective QTLs and/or transgenes into elite genotypes without detrimental effects on yield potential, which will lead to new cultivars with high yield potential and high yield stability, that will result in superior performance in dry environments.
Journal ArticleDOI
Hydrogen peroxide signalling
TL;DR: Signalling roles for hydrogen peroxide during abscisic-acid-mediated stomatal closure, auxin-regulated root gravitropism and tolerance of oxygen deprivation are now evident.
Journal ArticleDOI
Cell death by necrosis: towards a molecular definition
TL;DR: Because necrosis is prominent in ischemia, trauma and possibly some forms of neurodegeneration, further biochemical comprehension and molecular definition of this process could have important clinical implications.
Journal ArticleDOI
Redox Regulation: A Broadening Horizon
Bob B. Buchanan,Yves Balmer +1 more
TL;DR: Research in areas currently under exploration promises to provide a fuller understanding of the role redox plays in cellular processes, and to further the application of this knowledge to technology and medicine.
References
More filters
Journal ArticleDOI
Mitochondria and apoptosis
Douglas R. Green,John C. Reed +1 more
TL;DR: A variety of key events in apoptosis focus on mitochondria, including the release of caspase activators (such as cytochrome c), changes in electron transport, loss of mitochondrial transmembrane potential, altered cellular oxidation-reduction, and participation of pro- and antiapoptotic Bcl-2 family proteins.
Journal ArticleDOI
The oxidative burst in plant disease resistance
TL;DR: Emerging data indicate that the oxidative burst reflects activation of a membrane-bound NADPH oxidase closely resembling that operating in activated neutrophils, which underlies the expression of disease-resistance mechanisms.
Journal ArticleDOI
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
TL;DR: It is reported here that H2O2 from this oxidative burst not only drives the cross-linking of cell wall structural proteins, but also functions as a local trigger of programmed death in challenged cells and as a diffusible signal for the induction in adjacent cells of genes encoding cellular protectants.
Related Papers (5)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Programmed cell death, mitochondria and the plant hypersensitive response
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction
Klaus Apel,Heribert Hirt +1 more