Journal ArticleDOI
Parthenogenetic activation of oocytes in c-mos-deficient mice
Naohiro Hashimoto,Nobumoto Watanabe,Yasuhide Furuta,Hiroyuki Tamemoto,Noriyuiki Sagata,Minesuke Yokoyama,Kenji Okazaki,Mariko Nagayoshi,Naoki Takeda,Yoji Ikawa,Shinichi Aizawa +10 more
TLDR
Results indicate that in mice Mos plays a role in the second meiotic metaphase arrest, but does not seem to be essential for the initiation of oocyte maturation, spermatogenesis or somatic cell cycle.Abstract:
IN Xenopus the c-mos proto-oncogene product (Mos) is essential for the initiation of oocyte maturation1, for the progression from meiosis I to meiosis II2,3 and for the second meiotic metaphase arrest, acting as an essential component of the cytostatic factor CSF4,5. Its function in mouse oocytes is unclear6–9, however, as is the biological significance of c-mos mRNA expression in testes1,10 and several somatic tissues1,10,11. We have generated c-mos-deficient mice by gene targeting in embryonic stem cells. These mice grew at the same rate as their wild-type counterparts and reproduction was normal in the males, but the fertility of the females was very low. The c-mos-deficient female mice developed ovarian teratomas at a high frequency. Oocytes from these females matured to the second meiotic metaphase both in vivo and in vitro, but were activated without fertilization. The results indicate that in mice Mos plays a role in the second meiotic metaphase arrest, but does not seem to be essential for the initiation of oocyte maturation, spermatogenesis or somatic cell cycle.read more
Citations
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Book ChapterDOI
Signal transduction through MAP kinase cascades.
TL;DR: The chapter explores the cellular substrates of MAP kinases, wherein it discusses about protein kinase substrates for MAPKS, nuclear transcription factors, signaling components, and cytoskeletal proteins.
Journal ArticleDOI
Derivation of oocytes from mouse embryonic stem cells
Karin Hübner,Guy Fuhrmann,Lane K. Christenson,James Kehler,Rolland Reinbold,Rabindranath De La Fuente,Jennifer R. Wood,Jerome F. Strauss,Michele Boiani,Hans R. Schöler +9 more
TL;DR: It is shown that mouse embryonic stem cells in culture can develop into oogonia that enter meiosis, recruit adjacent cells to form follicle-like structures, and later develop into blastocysts.
Journal ArticleDOI
Specific interference with gene function by double-stranded RNA in early mouse development
TL;DR: It is shown that dsRNA is effective as a specific inhibitor of the function of three genes in the mouse, namely maternally expressed c-mos in the oocyte and zygotically expressed E-cadherin or a GFP transgene in the preimplantation embryo.
Journal ArticleDOI
Nutrient-dependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila.
TL;DR: A causal link between nutrient availability and insulin-dependent growth is established and results point to a conserved role of the neuroendocrine axis in growth control in multicellular organisms.
Journal ArticleDOI
Genomic imprinting disrupted by a maternal effect mutation in the Dnmt1 gene
Carina Howell,Timothy H. Bestor,Feng Ding,Keith E. Latham,Carmen Mertineit,Jacquetta M. Trasler,J. Richard Chaillet +6 more
TL;DR: Transient nuclear localization of Dnmt1o in 8-cell embryos suggests that this variant of DNmt1 provides maintenance methyltransferase activity specifically at imprinted loci during the fourth embryonic S phase.
References
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Journal ArticleDOI
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Yoshio Masui,Clement L. Markert +1 more
TL;DR: Fully grown oocytes of the frog (Rana pipiens) undergo cytoplasmic and nuclear maturation when treated with progesterone after the follicular envelopes have been removed, and the arrest of mitosis and cleavage can be attributed to a specific “cytostatic factor” in the cy toplasm of the secondary oocyte.
Journal ArticleDOI
The c- mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs
TL;DR: Results demonstrate that Mos protein is the cytostatic factor CSF, long known as an endogenous meiotic inhibitor in vertebrate eggs, and that egg cytosol extracts, when immunodepleted of endogenous pp39mos, lose their cleavage-arresting activity in injected embryos.
Journal ArticleDOI
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes
TL;DR: The c-mos proto-oncogene is expressed as a maternal mRNA in oocytes and early embryos of Xenopus laevis, but its translation product pp39mos is detectable only during progesterone-induced oocyte maturation, indicating that it functions during reinitiation of meiotic division.