Plasma lipoprotein metabolism in perfused rat livers. ii. transfer of free and esterified cholesterol into the plasma

TLDR: When cholesterol was a dietary component (2% of the diet), it was dissolved in olive oil and fed for 5 to 7 days, except in the experiments described in Tables VI, VII, and VIII; in those, cholesterol was fed for 12 to 14 days in order to produce livers of very high lipid content.

Abstract: When cholesterol was a dietary component (2% of the diet), it was dissolved in olive oil and fed for 5 to 7 days, except in the experiments described in Tables VI, VII, and VIII; in those, cholesterol was fed for 12 to 14 days in order to produce livers of very high lipid content. Liver perfusion. The technique of perfusion of Miller and associates (6, 7) was used. Mevalonic acid-2-C'4 was used as sterol precursor. In some experiments, 3.3 to 5.5 ,sc (0.7 to 1.2 mg) was added directly to the perfusing blood; one-third of the total was added at the start of the experiment, and the rest, by continuous injection during the perfusion (2 to 4 hours). In other experiments, designed to study the transfer of labeled cholesterol from liver to plasma, 11 ,uc of mevalonic acid-2-C"4 was given in two ip injections to the rats used as liver donors, at 16 hours and 3 hours before removal of their livers.